Evidence map›Paper›PMID 36210702›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2022

[miRNA-26a reduces vascular smooth muscle cell calcification by regulating connective tissue growth factor].

W Wu, L Cheng, J Wang, C Yang, Y Shang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

W WuDepartment of Cardiac and Vascular Surgery, Central Hospital of Wuhan, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430000, China.
L ChengDepartment of Cardiac and Vascular Surgery, Central Hospital of Wuhan, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430000, China.
J WangDepartment of Cardiac and Vascular Surgery, Central Hospital of Wuhan, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430000, China.
C YangDepartment of Cardiac and Vascular Surgery, Central Hospital of Wuhan, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430000, China.
Y ShangDepartment of Cardiac and Vascular Surgery, Central Hospital of Wuhan, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430000, China.
Huazhong University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the regulatory role of miRNA-26a in vascular smooth muscle cell (VSMC) calcification by regulating connective tissue growth factor (CTGF).

methodsRat thoracic aorta VSMCs (A7r5 cells) with induced calcification were treated with AR234960 agonist or transfected with miR-26a mimic, or with both treatments. Alizarin red staining was used to determine calcium deposition, and phosphatase (ALP) activity in the cells was measured. The mRNA and protein expressions of miR-26a, OPG, OPN, BMP-2 and collagen Ⅱ were detected using qPCR and Western blotting. The binding of miR-26a to CTGF was verified using dual luciferase reporter gene assay.

resultsAfter induced calcification, A7r5 cells showed gradually decreased miR-26a expression (

conclusionmiRNA-26a can effectively alleviate vascular calcification by lowering the level of CTGF, reducing ALP activity and the expressions of OPN, BMP-2 and collagen Ⅱ, and increasing the expression of OPG.

Indexed as

MicroRNAsVascular CalcificationAnimalsCalciumCells, CulturedConnective Tissue Growth FactorMuscle, Smooth, VascularMyocytes, Smooth MusclePhosphoric Monoester HydrolasesRatsRNA, MessengerSulfonesAR234960CalciumConnective Tissue Growth FactorMicroRNAsMIRN26b microRNA, ratPhosphoric Monoester HydrolasesRNA, MessengerSulfonesconnective tissue growth factormiRNA-26avascular calcification

Identifiers

PMID36210702
PMCPMC9550542
OpenAlexW4304116874

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.