Evidence map›Paper›PMID 36247259›Full record

ArticleAmerican journal of translational research2022

RNA expression profiling from the liquid fraction of synovial fluid in knee joint osteoarthritis patients.

Peng Jiang, Shui Sun, Ju Zhang, Cuidan Li, Guannan Ma, Jian Wang, Fei Chen, Dezhong Joshua Liao

Abstract read
PubMed Full text at PMC
In one paragraph

Article in American journal of translational research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. The Role of AIM2 Inflammasome in Knee Osteoarthritis.Journal of inflammation research · 2022
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Peng JiangSchool of Clinical Medicine, Shandong University Jinan 250100, Shandong Province, China.
Shui SunSchool of Clinical Medicine, Shandong University Jinan 250100, Shandong Province, China.
Ju ZhangCAS Key Laboratory of Genomics Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences Beijing 100101, China.
Cuidan LiCAS Key Laboratory of Genomics Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences Beijing 100101, China.
Guannan MaCAS Key Laboratory of Genomics Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences Beijing 100101, China.
Jian WangDepartment of Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong Province, China.
Fei ChenCAS Key Laboratory of Genomics Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences Beijing 100101, China.
Dezhong Joshua LiaoDepartment of Pathology, and Key Lab of Endemic and Ethnic Diseases of the Ministry of Education of China in Guizhou Medical University Guiyang 550004, Guizhou Province, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the RNA profile of synovial fluid (SF) from osteoarthritis (OA) patients and carry out cluster analysis of OA-related genes.

methodsRNA of SF from OA patients was isolated using RNA-specific Trizol. A cDNA library was built and subjected to the second-generation sequencing using HisSeq4000 with a data size of 8G. The sequencing reads were aligned to the UCSC human reference genome (hg38) using Tophat with default parameters. Gene function enrichment was generated using DAVID.

resultsThe minimum weight 0.096 µg RNA of SF sample was used for sequencing analysis, which produced 66,154,562 clean reads, 91.28% of which were matched to the reference with 2,682 genes identified. Some of the unmatchable reads matched RNAs of bacteria, mainly

conclusionThese results suggest that less than 0.1 µg RNA is sufficient for establishing a cDNA library and deep sequencing, and that the liquid fraction of SF contains a whole RNA repertoire that may reflect a history of previous microorganism infections.

Indexed as

knee jointOsteoarthritissecond-generation sequencingsynovial fluidtranscriptome

Identifiers

PMID36247259
PMCPMC9556501

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.