Evidence map›Paper›PMID 36255669›Full record

ArticleHead and neck pathology2023

Epstein-Barr Virus Detection and LMP-1 Expression in Thai Patients with Oral Squamous Cell Carcinoma.

Dulyapong Rungraungrayabkul, Puangwan Lapthanasupkul, Nakarin Kitkumthorn, Jintana Pankam, Boworn Klongnoi, Siribang-On Piboonniyom Khovidhunkit

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Head and neck pathology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Dulyapong RungraungrayabkulDepartment of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand.
Puangwan LapthanasupkulDepartment of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand.
Nakarin KitkumthornDepartment of Oral Biology, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand.
Jintana PankamDevelopment of Disease Management Model for Oral Cancer With an Integration Network of Screening, Surveillance, and Treatment From Primary Care Unit to Tertiary Care in Nakhonratchasima Province Project, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand.
Boworn KlongnoiDevelopment of Disease Management Model for Oral Cancer With an Integration Network of Screening, Surveillance, and Treatment From Primary Care Unit to Tertiary Care in Nakhonratchasima Province Project, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand.
Siribang-On Piboonniyom KhovidhunkitDevelopment of Disease Management Model for Oral Cancer With an Integration Network of Screening, Surveillance, and Treatment From Primary Care Unit to Tertiary Care in Nakhonratchasima Province Project, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand. Siribangon.pib@mahidol.edu.ORCID http://orcid.org/0000-0002-4496-7964
Mahidol University · TH

Funding

Mahidol University 051/2562
6 · The paper itself

Abstract

backgroundTo date, high-risk human papillomavirus (HPV) is primarily linked to oropharyngeal carcinoma, but only a small subset of oral squamous cell carcinoma (OSCC) is truly driven by high-risk HPV. In addition, Epstein-Barr virus (EBV) is another potential oncogenic virus for OSCC development. This study aims to investigate the role of EBV infection in Thai patients with OSCC.

methodsForty-seven formalin-fixed paraffin-embedded specimens of OSCC were obtained. EBV DNA was detected by polymerase chain reaction analysis using primers for LMP-1 region of EBV. EBV-positive OSCC cases were subjected to LMP-1 immunohistochemical analysis and EBV-encoded small RNA (EBER) in situ hybridization to determine EBV cellular localization in OSCC. LMP-1 immunohistochemical analysis was also performed in all EBV-negative OSCC cases.

resultsOf the 47 OSCC specimens, ten (21%) exhibited EBV DNA by PCR analysis. Seven of ten (70%) EBV-positive specimens showed high-grade LMP-1 expression by immunohistochemistry. However, no EBER expression was detected in all EBV-positive OSCC specimens. In EBV-negative specimens, LMP-1 was also negative except in 3 specimens which showed low grade expression of LMP-1.

conclusionThe prevalence of EBV infection in OSCC in this group of Thai patients was 21%. Most of EBV-positive OSCC cases showed LMP-1 expression but a lack of EBER expression. From our findings, we presume that EBV may take some roles in OSCC development in this group of participants.

Indexed as

Epstein-Barr Virus InfectionsMouth NeoplasmsSquamous Cell Carcinoma of Head and NeckHerpesvirus 4, HumanHumansSoutheast Asian PeopleViral Matrix ProteinsEBV-associated membrane antigen, Epstein-Barr virusViral Matrix ProteinsEBEREBVLMP-1Oral cancerOSCCPCRThai

Identifiers

PMID36255669
PMCPMC10063726
OpenAlexW4306694246

What Socratic holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.