Evidence mapPaperPMID 36284018Full record

ReviewAnalytical and bioanalytical chemistry2023

Advancement and obstacles in microfluidics-based isolation of extracellular vesicles.

Megan Havers, Axel Broman, Andreas Lenshof, Thomas Laurell

Open access · hybridAbstract readReview
In one paragraph

Review in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
4.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 63 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Megan HaversBiomedical Engineering Department, Lund University, Ole Römers väg 3, 221 00, Lund, Sweden. megan.havers@bme.lth.se.
Axel BromanBiomedical Engineering Department, Lund University, Ole Römers väg 3, 221 00, Lund, Sweden. axel.broman@bme.lth.se.
Andreas LenshofBiomedical Engineering Department, Lund University, Ole Römers väg 3, 221 00, Lund, Sweden.
Thomas LaurellBiomedical Engineering Department, Lund University, Ole Römers väg 3, 221 00, Lund, Sweden.
Lund University · SE

Funding

The European Commission 80127Vetenskapsrådet 2019-00795
6 · The paper itself

Abstract

There is a great need for techniques which enable reproducible separation of extracellular vesicles (EVs) from biofluids with high recovery, purity and throughput. The development of new techniques for isolation of EVs from minute sample volumes is instrumental in enabling EV-based biomarker profiling in large biobank cohorts and paves the way to improved diagnostic profiles in precision medicine. Recent advances in microfluidics-based devices offer a toolbox for separating EVs from small sample volumes. Microfluidic devices that have been used in EV isolation utilise different fundamental principles and rely largely on benefits of scaling laws as the biofluid processing is miniaturised to chip level. Here, we review the progress in the practicality and performance of both passive devices (such as mechanical filtering and hydrodynamic focusing) and active devices (using magnetic, electric or acoustic fields). As it stands, many microfluidic devices isolate intact EV populations at higher purities than centrifugation, precipitation or size-exclusion chromatography. However, this comes at a cost. We address challenges (in particular low throughput, clogging risks and ability to process biofluids) and highlight the need for more improvements in microfluidic devices. Finally, we conclude that there is a need to refine and standardise these lab-on-a-chip techniques to meet the growing interest in the diagnostic and therapeutic value of purified EVs.

Indexed as

Extracellular VesiclesMicrofluidicsChromatography, GelHydrodynamicsLab-On-A-Chip DevicesExtracellular vesiclesMicrofluidicsNanoparticle isolation

Identifiers

PMID36284018
PMCPMC9928917
OpenAlexW4307249118

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.