ArticleeLife2022
Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
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- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy.Npj viruses · 2026Review
- Neural evolution of complex motor behaviors: insights from Drosophila courtship song.Current opinion in neurobiology · 2025Review
- High-resolution single-cell analyses reveal evolutionary constraints and evolvability of sexual circuits inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Synaptic MEMOIR: mapping individual synapses of neurons with protein barcodes.bioRxiv : the preprint server for biology · 2025Article
- An essential experimental control for functional connectivity mapping with optogenetics.Genetics · 2025Article
- A neural circuit for context-dependent multimodal signaling in Drosophila.Nature communications · 2025Article
- Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning.Science (New York, N.Y.) · 2025Article
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- Gradients of Cell Recognition Molecules Wire Visuomotor Transformation.bioRxiv : the preprint server for biology · 2025Article
- Comparative prospects of imaging methods for whole-brain mammalian connectomics.Cell reports methods · 2025Review
- Expansion microscopy reveals neural circuit organization in genetic animal models.Neurophotonics · 2025Review
- Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy.Nature communications · 2024Article
- Image processing tools for petabyte-scale light sheet microscopy data.Nature methods · 2024Article
- Article
Corrections and comments
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Authors and funding
19 authors at 2 institutions in 3 countries.
Funding
Abstract
Brain function is mediated by the physiological coordination of a vast, intricately connected network of molecular and cellular components. The physiological properties of neural network components can be quantified with high throughput. The ability to assess many animals per study has been critical in relating physiological properties to behavior. By contrast, the synaptic structure of neural circuits is presently quantifiable only with low throughput. This low throughput hampers efforts to understand how variations in network structure relate to variations in behavior. For neuroanatomical reconstruction, there is a methodological gulf between electron microscopic (EM) methods, which yield dense connectomes at considerable expense and low throughput, and light microscopic (LM) methods, which provide molecular and cell-type specificity at high throughput but without synaptic resolution. To bridge this gulf, we developed a high-throughput analysis pipeline and imaging protocol using tissue expansion and light sheet microscopy (ExLLSM) to rapidly reconstruct selected circuits across many animals with single-synapse resolution and molecular contrast. Using
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.