Evidence map›Paper›PMID 36296237›Full record

ReviewMicroorganisms2022

Beyond Basic Diversity Estimates-Analytical Tools for Mechanistic Interpretations of Amplicon Sequencing Data.

Anna Trego, Ciara Keating, Corine Nzeteu, Alison Graham, Vincent O'Flaherty, Umer Zeeshan Ijaz

Abstract readReview
In one paragraph

Review in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Anna TregoMicrobial Ecology Laboratory, School of Biological and Chemical Sciences and the Ryan Institute, University of Galway, University Road, H91 TK33 Galway, Ireland.ORCID 0000-0002-2797-7538
Ciara KeatingInstitute of Biodiversity, Animal Health & Comparative Medicine, The University of Glasgow, Oakfield Avenue, Glasgow G12 8LT, UK.ORCID 0000-0001-9199-3068
Corine NzeteuMicrobial Ecology Laboratory, School of Biological and Chemical Sciences and the Ryan Institute, University of Galway, University Road, H91 TK33 Galway, Ireland.ORCID 0000-0003-3225-9810
Alison GrahamMicrobial Ecology Laboratory, School of Biological and Chemical Sciences and the Ryan Institute, University of Galway, University Road, H91 TK33 Galway, Ireland.
Vincent O'FlahertyMicrobial Ecology Laboratory, School of Biological and Chemical Sciences and the Ryan Institute, University of Galway, University Road, H91 TK33 Galway, Ireland.
Umer Zeeshan IjazWater Engineering Group, School of Engineering, The University of Glasgow, Oakfield Avenue, Glasgow G12 8LT, UK.ORCID 0000-0001-5780-8551

Funding

Engineering and Physical Sciences Research Council EP/P029329/1Engineering and Physical Sciences Research Council EP/V030515/1Enterprise Ireland TC/2014/0016Natural Environment Research Council NE/L011956/1Science Foundation Ireland 14/IA/2371Science Foundation Ireland 16/RC/3889
6 · The paper itself

Abstract

Understanding microbial ecology through amplifying short read regions, typically 16S rRNA for prokaryotic species or 18S rRNA for eukaryotic species, remains a popular, economical choice. These methods provide relative abundances of key microbial taxa, which, depending on the experimental design, can be used to infer mechanistic ecological underpinnings. In this review, we discuss recent advancements in in situ analytical tools that have the power to elucidate ecological phenomena, unveil the metabolic potential of microbial communities, identify complex multidimensional interactions between species, and compare stability and complexity under different conditions. Additionally, we highlight methods that incorporate various modalities and additional information, which in combination with abundance data, can help us understand how microbial communities respond to change in a typical ecosystem. Whilst the field of microbial informatics continues to progress substantially, our emphasis is on popular methods that are applicable to a broad range of study designs. The application of these methods can increase our mechanistic understanding of the ongoing dynamics of complex microbial communities.

Indexed as

16S rRNAampliconsecologymicrobiomesequence analysis

Identifiers

PMID36296237
PMCPMC9609705

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.