Evidence map›Paper›PMID 36303448›Full record

ArticleEuropean journal of immunology2023

Guidelines for mouse and human DC generation.

Manfred B Lutz, Shafaqat Ali, Cindy Audiger, Stella E Autenrieth, Luciana Berod, Venetia Bigley, Laura Cyran, Marc Dalod, Jan Dörrie, Diana Dudziak and 16 more

Open access · hybridAbstract read
In one paragraph

Article in European journal of immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 48 citations in OpenAlex.

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  7. GM-CSF Promotes Superior In Vitro Differentiation of MHC IIInternational journal of molecular sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors at 18 institutions in 6 countries.

Manfred B LutzInstitute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.
Shafaqat AliInstitute of Medical Microbiology and Hospital Hygiene, University of Düsseldorf, Düsseldorf, Germany.
Cindy AudigerImmunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
Stella E AutenriethDendritic Cells in Infection and Cancer (F171), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Luciana BerodInstitute of Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, 55131, Germany.
Venetia BigleyTranslational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, NE2 4HH, United Kingdom.
Laura CyranInstitute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.
Marc DalodCNRS, INSERM, Aix Marseille Univ, Centre d'Immunologie de Marseille-Luminy, Turing Center for Living Systems, Marseille, France.
Jan DörrieRNA-based Immunotherapy, Hautklinik, Universitätsklinikum Erlangen (UKER), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany.
Diana DudziakComprehensive Cancer Center Erlangen European Metropolitan Area of Nuremberg (CCC ER-EMN), Östliche Stadtmauerstraße 30, 91054, Erlangen, Germany.
Georgina Flórez-GrauDepartment of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands.
Lucila GiusianoInstitute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, 55131, Germany.
Gloria J GodoyInstitute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, 55131, Germany.
Marion HeuerInstitute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.
Anne B KrugInstitute for Immunology, Biomedical Center, Faculty of Medicine, Ludwig-Maximilians-University Munich, Planegg-Martinsried, Germany.
Christian H K LehmannLaboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany.
Christian T MayerExperimental Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Shalin H NaikImmunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
Stefanie ScheuInstitute of Medical Microbiology and Hospital Hygiene, University of Düsseldorf, Düsseldorf, Germany.
Gerty SchreibeltDepartment of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands.
Elodie SeguraInstitut Curie, PSL Research University, INSERM, U932, 26 rue d'Ulm, Paris, 75005, France.
Kristin SeréInstitute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany.
Tim SparwasserInstitute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, 55131, Germany.
Jurjen TelLaboratory of Immunoengineering, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Huaming XuInstitute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany.
Martin ZenkeInstitute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany.
RWTH Aachen University · DEUniversity of Würzburg · DEHeinrich Heine University Düsseldorf · DERadboud Institute for Molecular Life Sciences · NLUniversity Medical Center of the Johannes Gutenberg University Mainz · DEUniversity of Melbourne · AUCentre d’Immunologie de Marseille-Luminy · FRComprehensive Cancer Center Erlangen · DEEindhoven University of Technology · NLGerman Climate Computing Centre · DEInstitute of Medical Microbiology and Hygiene · DEJohannes Gutenberg University Mainz · DELudwig-Maximilians-Universität München · DENational Institutes of Health · USNewcastle University · GBUniversitätsklinikum Erlangen · DEUniversité Paris Sciences et Lettres · FRUniversity of Tübingen · DE

Funding

The role of apoptosis in B lymphocyte biologyZIABC011975 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI MAYER, CHRISTIAN · 2020 to 2025
$5.2M
Intramural NIH HHS ZIA BC011975Medical Research Council MR/W01677X/1Wellcome TrustWellcome Trust 101155/Z/13/Z
6 · The paper itself

Abstract

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non-lymphoid tissues. This article provides protocols with top ticks and pitfalls for preparation and successful generation of mouse and human DC from different cellular sources, such as murine BM and HoxB8 cells, as well as human CD34

Indexed as

Dendritic CellsMonocytesAnimalsAntigens, CD34Cell DifferentiationHumansMicePhenotypeAntigens, CD34Dendritic cellsGenerationIn vitroIsolation

Identifiers

PMID36303448
PMCPMC10704271
OpenAlexW4307496533

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.