ArticleMolecular ecology resources2023
Development of epigenetic biomarkers for the identification of sex and thermal stress in fish using DNA methylation analysis and machine learning procedures.
Article in Molecular ecology resources, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Gene Expression-Based Classification of European Seabass Larval Batches According to Saddleback Syndrome Incidence Using Machine Learning.Animals : an open access journal from MDPI · 2026Article
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- Integrated Transcriptome and Methylome Analyses Reveal Sex-Specific Molecular Responses to Chronic Heat Stress in Tongue Sole (Animals : an open access journal from MDPI · 2026Article
- The potential of methylation signal detection in eDNA toward functional ecological monitoring.Communications biology · 2026Review
- Genome-Wide Characterization of Sex-Linked Regions in the Sangzhi Horned Toad (Boulenophrys sangzhiensis) Reveals Complex Sex Determination Mechanisms.Molecular ecology resources · 2026Article
- Genome-Wide DNA Methylation Patterns Predict Age in the Zebra Shark (Stegostoma tigrinum) and Provide Insight Into the Evolution of Vertebrate Aging.Molecular ecology · 2026Article
- Genetic and environmental drivers of sex determination in turtles: insights and implications for conservation.Molecular biology reports · 2026Review
- Teleosts as Models for Epigenetic Inheritance.Molecular ecology · 2026Review
- Epigenetics in Captivity: Restoring Wild Phenotypes in Captive-Reared Salmonids.Evolutionary applications · 2026Article
- Effects of Black Soldier Fly Larval (Aquaculture nutrition · 2026Article
- Review
- Sex differences in DNA methylation in bats.Annals of the New York Academy of Sciences · 2025Article
- Genome-Wide Identification of DNA Methyltransferases (Dnmts) in Fish and Its Potential Roles During Sex Change in Blackhead Seabream.Biomolecules · 2025Article
- Oysters in transition: hermaphrodite oysters display unique DNA methylation patterns in gill tissue.BMC genomics · 2025Article
- Environmental DNA Epigenetics Accurately Predicts the Age of Cultured Fish Larvae.Ecology and evolution · 2025Article
- A cost-effective method for combining the power of genetic and epigenetic selection in animal production.Environmental epigenetics · 2025Article
- Exploring Multifunctional Markers of Biological Age in Farmed Gilthead Sea Bream (International journal of molecular sciences · 2024Article
- An epigenetic toolbox for conservation biologists.Evolutionary applications · 2024Article
- Genomics for monitoring and understanding species responses to global climate change.Nature reviews. Genetics · 2024Review
- Exploring the Effects of Rearing Densities on Epigenetic Modifications in the Zebrafish Gonads.International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
The sex ratio is a key ecological demographic parameter crucial for population viability. However, the epigenetic mechanisms operating during gonadal development regulating gene expression and the sex ratio remain poorly understood. Moreover, there is interest in the development of epigenetic markers associated with a particular phenotype or as sentinels of environmental effects. Here, we profiled DNA methylation and gene expression of 10 key genes related to sex development and stress, including steroidogenic enzymes, and growth and transcription factors. We provide novel information on the sex-related differences and on the influence of elevated temperature on these genes in zebrafish, a species with mixed genetic and environmental influences on sex ratios. We identified both positive (e.g., amh, cyp11c and hsd11b2) and negative (e.g., cyp11a1 and dmrt1) correlations in unexposed males, and negative correlation (amh) in exposed females between DNA methylation and gene expression levels. Further, we combined DNA methylation analysis with machine learning procedures and found a series of informative CpGs capable not only of correctly identifying sex (based on cyp19a1a DNA methylation levels) but also of identifying whether males and females had been exposed to abnormally elevated temperature when young (based on amh and foxl2a DNA methylation levels, respectively). This was achieved in the absence of conspicuous morphological alterations of the gonads. These DNA methylation-based epigenetic biomarkers represent molecular resources that can correctly recapitulate past thermal history and pave the way for similar findings in other species to assess potential ecological effects of environmental disturbances in the context of climate change.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.