Evidence map›Paper›PMID 36329583›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2022

[P2X7R promotes migration and invasion of Lewis lung cancer cells by activating the AKT signaling pathway].

Y Tang, R Zhao, C Qiao, X Li, X Bai, X Peng

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Y TangSchool of Medical Laboratory, Weifang Medical University, Key Laboratory of Clinical Laboratory Diagnostics of Shandong Province, Weifang 261053, China.
R ZhaoSchool of Medical Laboratory, Weifang Medical University, Key Laboratory of Clinical Laboratory Diagnostics of Shandong Province, Weifang 261053, China.
C QiaoSchool of Medical Laboratory, Weifang Medical University, Key Laboratory of Clinical Laboratory Diagnostics of Shandong Province, Weifang 261053, China.
X LiSchool of Medical Laboratory, Weifang Medical University, Key Laboratory of Clinical Laboratory Diagnostics of Shandong Province, Weifang 261053, China.
X BaiSchool of Medical Laboratory, Weifang Medical University, Key Laboratory of Clinical Laboratory Diagnostics of Shandong Province, Weifang 261053, China.
X PengSchool of Medical Laboratory, Weifang Medical University, Key Laboratory of Clinical Laboratory Diagnostics of Shandong Province, Weifang 261053, China.
Weifang Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the role of P2X7 receptor (P2X7R) in migration and invasion of mouse Lewis lung cancer (LLC) cells and examine the tumorigenic ability of LLC cells in P2X7R-knockout mice.

methodsRT-PCR was used to examine P2X7R mRNA expression in LLC cells. LLC cells were treated with ATP (as a P2X7R agonist) or 2'- 3'- O- (4-benzoyl- benzoyl)-ATP (BzATP) (a P2X7R agonist) with or without pretreatment with P2X7R antagonist oxATP or A438079. The changes in migration and invasive abilities of the cells were evaluated using wound healing assay and Transwell assay; Western blotting was performed to determine the activation level of the key proteins in the AKT signaling pathway. The effects of BzATP, A438079, and LY294002 (a inhibitor of the PI3K/AKT pathway) on migration and invasion of LLC cells were also examined. In wild-type (WT) and P2X7R knockout (P2X7

resultsP2X7R expression was detected in LLC cells. Treatment with P2X7R agonist significantly enhanced migration and invasion abilities of LLC cells, and this effect was inhibited by application of P2X7R antagonists (

conclusionP2X7R promotes migration and invasion of LLC cells by activating the AKT signaling pathway, and LLC cells show lowered tumorigenic capacity in P2X7

Indexed as

Lung NeoplasmsReceptors, Purinergic P2X7Adenosine TriphosphateAnimalsHumansMiceMice, Inbred C57BLPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionAdenosine TriphosphatePhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktReceptors, Purinergic P2X7AKT signaling pathwayinvasionLewis lung cancermigrationP2X7 receptor

Identifiers

PMID36329583
PMCPMC9637501
OpenAlexW4308368566

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.