ArticleNature methods2022
Capturing the start point of the virus-cell interaction with high-speed 3D single-virus tracking.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Mapping Nanoscale Forces and Potentials in Live Cells with Microsecond 3D Single-Particle Tracking.Chemical & biomedical imaging · 2026Article
- Intrinsic OPTIR imaging and spectroscopy enable label-free monitoring of viral infection in living cells.Analytical and bioanalytical chemistry · 2026Article
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Hydrodynamic dispersion drives viral-cellular contact for gene delivery in porous media.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- High-speed 3D single-virus tracking reveals actin-aided viral trafficking of SARS-CoV-2 on the plasma membrane.bioRxiv : the preprint server for biology · 2026Article
- Discrete long-range on-cell motion of bacteriophage T4.Microbiology spectrum · 2026Article
- Volumetric Single-Molecule Tracking Inside Subcellular Structures.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A Mechanotransduction-Aware Strategy for Enhancing MSC Potency via 3D Culture and Localized Delivery.Cyborg and bionic systems (Washington, D.C.) · 2026Article
- Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025Article
- Super-Resolving Particle Diffusion Heterogeneity in Porous Hydrogels via High-Speed 3D Active-Feedback Single-Particle Tracking Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Single molecule spectrum dynamics imaging with 3D target-locking tracking.Nature communications · 2025Article
- Super-resolving particle diffusion heterogeneity in porous hydrogels via high-speed 3D active-feedback single-particle tracking microscopy.bioRxiv : the preprint server for biology · 2025Article
- Long axial range 3D single-particle tracking using birefringent substrates.Nature communications · 2025Article
- Deep learning-assisted analysis of single-particle tracking for automated correlation between diffusion and function.Nature methods · 2025Article
- First contact: an interdisciplinary guide into decoding H5N1 influenza virus interactions with glycosaminoglycans in 3D respiratory cell models.Frontiers in cellular and infection microbiology · 2025Review
- Label-Free Anti-Brownian Trapping of Single Nanoparticles in Solution.The journal of physical chemistry. C, Nanomaterials and interfaces · 2024Article
- Early Steps of Individual Multireceptor Viral Interactions Dissected by High-Density, Multicolor Quantum Dot Mapping in Living Cells.ACS nano · 2024Article
- Single-nanoparticle electrophoretic mobility determination and trapping using active-feedback 3D tracking.bioRxiv : the preprint server for biology · 2024Article
- 4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.Nature communications · 2024Article
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5 authors.
Funding
Abstract
The early stages of the virus-cell interaction have long evaded observation by existing microscopy methods due to the rapid diffusion of virions in the extracellular space and the large three-dimensional cellular structures involved. Here we present an active-feedback single-particle tracking method with simultaneous volumetric imaging of the live cell environment called 3D-TrIm to address this knowledge gap. 3D-TrIm captures the extracellular phase of the infectious cycle in what we believe is unprecedented detail. We report what are, to our knowledge, previously unobserved phenomena in the early stages of the virus-cell interaction, including skimming contact events at the millisecond timescale, orders of magnitude change in diffusion coefficient upon binding and cylindrical and linear diffusion modes along cellular protrusions. Finally, we demonstrate how this method can move single-particle tracking from simple monolayer culture toward more tissue-like conditions by tracking single virions in tightly packed epithelial cells. This multiresolution method presents opportunities for capturing fast, three-dimensional processes in biological systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.