Evidence mapPaperPMID 36361571Full record

ArticleInternational journal of molecular sciences2022

Pioglitazone Attenuates the Effects of Peripheral Inflammation in a Human In Vitro Blood-Brain Barrier Model.

Gustavo Henrique Oliveira da Rocha, Rodrigo Azevedo Loiola, Marina de Paula-Silva, Fumitaka Shimizu, Takashi Kanda, Andrea Vieira, Fabien Gosselet, Sandra Helena Poliselli Farsky

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 21 citations in OpenAlex.

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  11. Inflammation in the CNS and PNS: From Molecular Basis to Therapy.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Gustavo Henrique Oliveira da RochaFaculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil.ORCID 0000-0001-9158-1194
Rodrigo Azevedo LoiolaLaboratoire de la Barrière Hémato-Encéphalique (LBHE), Faculté des Sciences Jean Perrin, Artois University, UR 2465, F-62300 Lens, France.
Marina de Paula-SilvaFaculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil.
Fumitaka ShimizuDepartment of Neurology and Clinical Neuroscience, Yamaguchi University, Ube 755-8505, Japan.ORCID 0000-0003-4163-2939
Takashi KandaDepartment of Neurology and Clinical Neuroscience, Yamaguchi University, Ube 755-8505, Japan.
Andrea VieiraFaculty of Medical Sciences, Clinic of Gastroenterology, Department of Medicine, Irmandade da Santa Casa de Misericórdia de São Paulo, São Paulo 01221-020, Brazil.
Fabien GosseletLaboratoire de la Barrière Hémato-Encéphalique (LBHE), Faculté des Sciences Jean Perrin, Artois University, UR 2465, F-62300 Lens, France.ORCID 0000-0002-0481-5026
Sandra Helena Poliselli FarskyFaculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil.
Universidade de São Paulo · BRUniversité d'Artois · FRYamaguchi University · JPIrmandade da Santa Casa de Misericórdia de São Paulo · BR

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior 88887.570112/2020-00ERANET JPcofuND 2-NET-PETABC 643417French National Agency ANR-20-JPW2-0002-04São Paulo Research Foundation 2017/05430-4
6 · The paper itself

Abstract

Biological mediators secreted during peripheral chronic inflammation reach the bloodstream and may damage the blood-brain barrier (BBB), triggering central nervous system (CNS) disorders. Full-fledged human BBB models are efficient tools to investigate pharmacological pathways and mechanisms of injury at the BBB. We here employed a human in vitro BBB model to investigate the effects of either plasma from inflammatory bowel disease (IBD) patients or tumor necrosis factor α (TNFα), a cytokine commonly released in periphery during IBD, and the anti-inflammatory role of pioglitazone, a peroxisome proliferator-activated receptor γ agonist (PPARγ). The BBB model was treated with either 10% plasma from healthy and IBD donors or 5 ng/mL TNFα, following treatment with 10 µM pioglitazone. Patient plasma did not alter BBB parameters, but TNFα levels in plasma from all donors were associated with varying expression of claudin-5, claudin-3 and ICAM-1. TNFα treatment increased BBB permeability, claudin-5 disarrangement, VCAM-1 and ICAM-1 expression, MCP1 secretion and monocyte transmigration. These effects were attenuated by pioglitazone. Plasma from IBD patients, which evoked higher BBB permeability, also increased ICAM-1 expression, this effect being reversed by pioglitazone. Our findings evidence how pioglitazone controls periphery-elicited BBB inflammation and supports its repurposing for prevention/treating of such inflammatory conditions.

Indexed as

Blood-Brain BarrierInflammatory Bowel DiseasesClaudin-5HumansInflammationIntercellular Adhesion Molecule-1PioglitazonePPAR gammaTumor Necrosis Factor-alphaClaudin-5Intercellular Adhesion Molecule-1PioglitazonePPAR gammaPPARG protein, humanTumor Necrosis Factor-alphablood–brain barrierinflammatory bowel diseaseperipheral inflammationpioglitazone

Identifiers

PMID36361571
PMCPMC9656730
OpenAlexW4307273321

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.