Evidence map›Paper›PMID 36398274›Full record

ArticleAmerican journal of translational research2022

miR-153-3p inhibits osteogenic differentiation of BMSCs by down-regulating the expression of RUNX2 in a high glucose environment.

Qinying Wang, Qian Xia, Maohua Meng, Ying Li, Zhenglong Tang, Xiao Zeng, Helin Chen, Jiayu Shu, Xingxing Xv, Jingqiao Chen and 5 more

Open access · greenAbstract read
In one paragraph

Article in American journal of translational research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Role of microRNA in Diabetic Osteoporosis.Molecular biotechnology · 2025
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Qinying WangSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Qian XiaSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Maohua MengSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Ying LiSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Zhenglong TangSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Xiao ZengSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Helin ChenSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Jiayu ShuSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Xingxing XvSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Jingqiao ChenSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Jing LuSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Huan WangSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Zhaoyang YeClinical Research Center, The Affiliated Hospital of Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Bin SongDepartment of Prosthodontics, Guizhou Provincial People's Hospital Guiyang 550003, Guizhou Province, China.
Qiang DongSchool of Stomatology, Guizhou Medical University Guiyang 550004, Guizhou Province, China.
Guiyang Medical University · CNGuizhou Provincial People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To study the effect of miR-153-3p on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in a high glucose environment and its potential mechanism. The results showed that high glucose inhibited the osteogenic differentiation of BMSCs, and the expression of miR-153-3p increased during osteogenic differentiation. Further experiments found that in BMSCs induced by high glucose, overexpression of miR-153-3p inhibited the osteogenic differentiation of BMSCs, and the expressions of osteogenesis-related genes bone sialoprotein, Collagen I and alkaline phosphatase were down-regulated, while silencing of miR-153-3p alleviated the inhibition effect. The dual-luciferase reporter gene assay confirmed that the 3'-untranslated region (3'-UTR) of runt related transcription factor 2 (RUNX2) had a targeted binding site with miR-153-3p and a negative regulatory effect. Molecular studies further confirmed that miR-153-3p inhibited the osteogenic differentiation of BMSCs by targeting the 3'-UTR of RUNX2. In conclusion, our study found that as one key regulator of high glucose affecting the osteogenic differentiation of BMSCs, miR-153-3p may play a negative regulatory role by inhibiting the expression of RUNX2.

Indexed as

BMSCshigh glucosemiR-153-3posteogenic differentiationRUNX2

Identifiers

PMID36398274
PMCPMC9641434
OpenAlexW4309411077

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.