Evidence map›Paper›PMID 36414713›Full record

ArticleMolecular psychiatry2024

Ketamine impairs growth cone and synaptogenesis in human GABAergic projection neurons via GSK-3β and HDAC6 signaling.

Xuan Li, Hexige Saiyin, Xinyu Chen, Qiong Yu, Lixiang Ma, Weimin Liang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular psychiatry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 13 citations in OpenAlex.

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  9. Local delivery of EGFRBioactive materials · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Xuan Li *Department of Anesthesiology, Huashan Hospital, Fudan University, Shanghai, China.
Hexige Saiyin *State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
Xinyu ChenDepartment of Anatomy and Histology & Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China.ORCID 0000-0002-1181-8188
Qiong YuDepartment of Anesthesiology, Huashan Hospital, Fudan University, Shanghai, China. yu_qiong816@sina.com.ORCID 0000-0003-3239-2734
Lixiang MaDepartment of Anatomy and Histology & Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China. lxma@fudan.edu.cn.
Weimin LiangDepartment of Anesthesiology, Huashan Hospital, Fudan University, Shanghai, China. chiefliang@sina.cn.
Fudan University · CNShanghai Medical College of Fudan University · CNSun Yat-sen University · CN

Funding

National Natural Science Foundation of China (National Science Foundation of China) 82171188National Natural Science Foundation of China (National Science Foundation of China) 82201329
6 · The paper itself

Abstract

The growth cone guides the axon or dendrite of striatal GABAergic projection neurons that protrude into the midbrain and cortex and form complex neuronal circuits and synaptic networks in a developing brain, aberrant projections and synaptic connections in the striatum related to multiple brain disorders. Previously, we showed that ketamine, an anesthetic, reduced dendritic growth, dendritic branches, and spine density in human striatal GABAergic neurons. However, whether ketamine affects the growth cone, the synaptic connection of growing striatal GABAergic neurons has not been tested. Using human GABAergic projection neurons derived from human inducible pluripotent stem cells (hiPSCs) and embryonic stem cells (ES) in vitro, we tested ketamine effects on the growth cones and synapses in developing GABAergic neurons by assessing the morphometry and the glycogen synthase kinase-3 (GSK-3) and histone deacetylase 6 (HDAC6) pathway. Ketamine exposure impairs growth cone formation, synaptogenesis, dendritic development, and maturation via ketamine-mediated activation of GSK-3 pathways and inhibiting HDAC6, an essential stabilizing protein for dendritic morphogenesis and synapse maturation. Our findings identified a novel ketamine neurotoxic pathway that depends on GSK-3β and HDAC6 signaling, suggesting that microtubule acetylation is a potential target for reducing ketamine's toxic effect on GABAergic projection neuronal development.

Indexed as

GABAergic NeuronsGlycogen Synthase Kinase 3 betaGrowth ConesHistone Deacetylase 6Induced Pluripotent Stem CellsKetamineNeurogenesisSignal TransductionSynapsesCorpus StriatumDendritesEmbryonic Stem CellsHumansGlycogen Synthase Kinase 3 betaHDAC6 protein, humanHistone Deacetylase 6Ketamine

Identifiers

PMID36414713
PMCPMC11371642
OpenAlexW4309681316

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.