Evidence map›Paper›PMID 36458321›Full record

ArticleThe Plant journal : for cell and molecular biology2023

A protocol for rapid generation cycling (speed breeding) of hemp (Cannabis sativa) for research and agriculture.

Susanne Schilling, Rainer Melzer, Caroline A Dowling, Jiaqi Shi, Shaun Muldoon, Paul F McCabe

Abstract read
In one paragraph

Article in The Plant journal : for cell and molecular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Genetic Advances inPlants (Basel, Switzerland) · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. The speed breeding technology of five generations per year in cotton.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Article
  13. Review
  14. Article
  15. Investigating the genetic control of plant development in spring barley under speed breeding conditions.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024
    Article
  16. Horticulture research · 2023
    Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Susanne Schilling *School of Biology and Environmental Science, University College Dublin, Belfield, Dublin, Ireland.ORCID 0000-0002-1142-6520
Rainer Melzer *School of Biology and Environmental Science, University College Dublin, Belfield, Dublin, Ireland.ORCID 0000-0003-0631-9023
Caroline A DowlingSchool of Biology and Environmental Science, University College Dublin, Belfield, Dublin, Ireland.ORCID 0000-0002-1184-7798
Jiaqi ShiSchool of Biology and Environmental Science, University College Dublin, Belfield, Dublin, Ireland.ORCID 0000-0003-2855-5385
Shaun MuldoonSchool of Biology and Environmental Science, University College Dublin, Belfield, Dublin, Ireland.
Paul F McCabeSchool of Biology and Environmental Science, University College Dublin, Belfield, Dublin, Ireland.ORCID 0000-0002-6146-8307

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hemp (Cannabis sativa) is a highly versatile crop with a multitude of applications, from textiles, biofuel and building material to high-value food products for consumer markets. Furthermore, non-hallucinogenic cannabinoids like cannabidiol (CBD), which can be extracted from female hemp flowers, are potentially valuable pharmacological compounds. In addition, hemp has high carbon sequestration potential associated with its rapid growth rate. Therefore, the hemp industry is gaining more traction and breeding hemp cultivars adapted to local climate conditions or bred for specific applications is becoming increasingly important. Here, we present a method for the rapid generation cycling (speed breeding) of hemp. The speed breeding protocol makes use of the photoperiod sensitivity of Cannabis. It encompasses vegetative growth of the plants for 2 weeks under continuous light, followed by 4 weeks under short-day conditions, during which flower induction, pollination and seed development proceed, and finally a seed ripening phase under continuous light and water stress. With the protocol described here, a generation time of under 9 weeks (61 days) from seed to seed can be achieved. Furthermore, our method synchronises the flowering time of different hemp cultivars, thus facilitating crosses between cultivars. The extremely short generation time will enable hemp researchers and breeders to perform crosses in a time-efficient way and generate new hemp cultivars with defined genetic characteristics over a short period of time.

Indexed as

CannabidiolCannabinoidsCannabisFlowersPlant BreedingCannabidiolCannabinoidscannabinoidsCannabis sativahempplant breedingrapid generation cyclingspeed breedingsustainabilitytechnical advance

Identifiers

PMID36458321
PMCPMC10108250

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.