Evidence map›Paper›PMID 36497004›Full record

ArticleCells2022

An Intracellular Metabolic Signature as a Potential Donor-Independent Marker of the Osteogenic Differentiation of Adipose Tissue Mesenchymal Stem Cells.

Daniela S C Bispo, Catarina S H Jesus, Katarzyna Romek, Inês M C Marques, Mariana B Oliveira, João F Mano, Ana M Gil

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Daniela S C BispoDepartment of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.ORCID 0000-0002-5813-5421
Catarina S H JesusDepartment of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.ORCID 0000-0003-1812-1663
Katarzyna RomekDepartment of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.
Inês M C MarquesDepartment of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.
Mariana B OliveiraDepartment of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.
João F ManoDepartment of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.ORCID 0000-0002-2342-3765
Ana M GilDepartment of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.ORCID 0000-0003-3766-4364
University of Aveiro · PT

Funding

European Research Council ERC-2019-ADG-883370, project REBORN (J.F.M.)Fundação para a Ciência e Tecnologia BIOIMPLANT project (PTDC/BTM-ORG/28835/2017) through the COMPETE2020 program and European Union fund FEDER (POCI-01-0145-FEDER-028835)Fundação para a Ciência e Tecnologia CEECIND/03605/2017Fundação para a Ciência e Tecnologia CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC)Fundação para a Ciência e Tecnologia National NMR Network (PTNMR) and, partially supported by Infrastructure Project Nº 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC)Fundação para a Ciência e Tecnologia PhD grant SFRH/BD/150655/2020 (and Sociedade Portuguesa de Química)
6 · The paper itself

Abstract

This paper describes an untargeted NMR metabolomics study to identify potential intracellular donor-dependent and donor-independent metabolic markers of proliferation and osteogenic differentiation of human adipose mesenchymal stem cells (hAMSCs). The hAMSCs of two donors with distinct proliferating/osteogenic characteristics were fully characterized regarding their polar endometabolome during proliferation and osteogenesis. An 18-metabolites signature (including changes in alanine, aspartate, proline, tyrosine, ATP, and ADP, among others) was suggested to be potentially descriptive of cell proliferation, independently of the donor. In addition, a set of 11 metabolites was proposed to compose a possible donor-independent signature of osteogenesis, mostly involving changes in taurine, glutathione, methylguanidine, adenosine, inosine, uridine, and creatine/phosphocreatine, choline/phosphocholine and ethanolamine/phosphocholine ratios. The proposed signatures were validated for a third donor, although they require further validation in a larger donor cohort. We believe that this proof of concept paves the way to exploit metabolic markers to monitor (and potentially predict) cell proliferation and the osteogenic ability of different donors.

Indexed as

Mesenchymal Stem CellsOsteogenesisAdipose TissueCell DifferentiationHumansPhosphorylcholinePhosphorylcholineadipose tissue mesenchymal stem cellscell proliferationdonor variabilityendometabolomemetabolomicsnuclear magnetic resonance (NMR) spectroscopyosteogenic differentiationpolar extracts

Identifiers

PMID36497004
PMCPMC9739047
OpenAlexW4310040187

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.