Evidence map›Paper›PMID 36497026›Full record

ArticleCells2022

Identification of the Inner Cell Mass and the Trophectoderm Responses after an In Vitro Exposure to Glucose and Insulin during the Preimplantation Period in the Rabbit Embryo.

Romina Via Y Rada, Nathalie Daniel, Catherine Archilla, Anne Frambourg, Luc Jouneau, Yan Jaszczyszyn, Gilles Charpigny, Véronique Duranthon, Sophie Calderari

Registry-linked trialAbstract read
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06064669 (Effect of Metformin on Healthy Live Birth After In-vitro Fertilization in Women With Prediabetes Mellitus), which is not on this map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT06064669 narecruitingstarted 2024, after this paper: background citation

Effect of Metformin on Healthy Live Birth After In-vitro Fertilization in Women With Prediabetes Mellitus: a Multicenter Double-blind Placebo Controlled Randomized Trial

Ran2024Enrolled988Registered outcomes26Posted comparisons0ConditionsIn-Vitro Fertilization, Metformin, PrediabetesArmsMetformin pretreatment before endometrial preparation for frozen embryo transfer, Metformin pretreatment before ovarian stimulation, Placebo pretreatment before endometrial preparation for frozen embryo transfer, Placebo pretreatment before ovarian stimulation
Open the trial in the graph
3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Genetic variant of the sheepAnimal biotechnology · 2024
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Romina Via Y RadaBREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Nathalie DanielBREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France.ORCID 0000-0002-2251-1296
Catherine ArchillaBREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Anne FrambourgBREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Luc JouneauBREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France.ORCID 0000-0002-5094-7302
Yan JaszczyszynInstitute for Integrative Biology of the Cell (I2BC), UMR 9198 CNRS, CEA, Paris-Sud University F, 91190 Gif-sur-Yvette, France.
Gilles CharpignyBREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France.ORCID 0000-0003-3954-7663
Véronique DuranthonBREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Sophie CalderariBREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France.ORCID 0000-0002-9669-681X

Funding

Agence Nationale de la Recherche ANR-10-LABX-73National Research Institute for Agriculture, Food and Environment PHASE-CI-VVF-2017Société Francophone du Diabète SFD-BD-2017
6 · The paper itself

Abstract

The prevalence of metabolic diseases is increasing, leading to more women entering pregnancy with alterations in the glucose-insulin axis. The aim of this work was to investigate the effect of a hyperglycemic and/or hyperinsulinemic environment on the development of the preimplantation embryo. In rabbit embryos developed in vitro in the presence of high insulin (HI), high glucose (HG), or both (HGI), we determined the transcriptomes of the inner cell mass (ICM) and the trophectoderm (TE). HI induced 10 differentially expressed genes (DEG) in ICM and 1 in TE. HG ICM exhibited 41 DEGs involved in oxidative phosphorylation (OXPHOS) and cell number regulation. In HG ICM, proliferation was decreased (p < 0.01) and apoptosis increased (p < 0.001). HG TE displayed 132 DEG linked to mTOR signaling and regulation of cell number. In HG TE, proliferation was increased (p < 0.001) and apoptosis decreased (p < 0.001). HGI ICM presented 39 DEG involved in OXPHOS and no differences in proliferation and apoptosis. HGI TE showed 16 DEG linked to OXPHOS and cell number regulation and exhibited increased proliferation (p < 0.001). Exposure to HG and HGI during preimplantation development results in common and specific ICM and TE responses that could compromise the development of the future individual and placenta.

Indexed as

GlucoseInsulinAnimalsBlastocystEmbryonic DevelopmentFemaleInsulin, Regular, HumanPregnancyRabbitsGlucoseInsulinInsulin, Regular, HumandiabetesDOHaDpreimplantation embryorabbit

Identifiers

PMID36497026
PMCPMC9736044

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.