Evidence map›Paper›PMID 36497121›Full record

ArticleCells2022

High-Content RNAi Phenotypic Screening Unveils the Involvement of Human Ubiquitin-Related Enzymes in Late Cytokinesis.

Mikaël Boullé, Laurianne Davignon, Keïs Nabhane Saïd Halidi, Salomé Guez, Emilie Giraud, Marcel Hollenstein, Fabrice Agou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Mikaël BoulléCenter for Technological Resources and Research (C2RT), Department of Structural Biology and Chemistry, Chemogenomic and Biological Screening Core Facility, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, F-75015 Paris, France.ORCID 0000-0002-6486-8533
Laurianne DavignonCenter for Technological Resources and Research (C2RT), Department of Structural Biology and Chemistry, Chemogenomic and Biological Screening Core Facility, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, F-75015 Paris, France.ORCID 0000-0003-4664-6854
Keïs Nabhane Saïd HalidiCenter for Technological Resources and Research (C2RT), Department of Structural Biology and Chemistry, Chemogenomic and Biological Screening Core Facility, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, F-75015 Paris, France.
Salomé GuezCenter for Technological Resources and Research (C2RT), Department of Structural Biology and Chemistry, Chemogenomic and Biological Screening Core Facility, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, F-75015 Paris, France.
Emilie GiraudCenter for Technological Resources and Research (C2RT), Department of Structural Biology and Chemistry, Chemogenomic and Biological Screening Core Facility, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, F-75015 Paris, France.ORCID 0000-0002-0028-1944
Marcel HollensteinLaboratory for Bioorganic Chemistry of Nucleic Acids, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, F-75015 Paris, France.ORCID 0000-0003-0263-9206
Fabrice AgouCenter for Technological Resources and Research (C2RT), Department of Structural Biology and Chemistry, Chemogenomic and Biological Screening Core Facility, Institut Pasteur, Université Paris Cité, CNRS UMR 3523, F-75015 Paris, France.
Centre National de la Recherche Scientifique · FR

Funding

Institut Carnot ARTS ANR-11-CNRT-04
6 · The paper itself

Abstract

CEP55 is a central regulator of late cytokinesis and is overexpressed in numerous cancers. Its post-translationally controlled recruitment to the midbody is crucial to the structural coordination of the abscission sequence. Our recent evidence that CEP55 contains two ubiquitin-binding domains was the first structural and functional link between ubiquitin signaling and ESCRT-mediated severing of the intercellular bridge. So far, high-content screens focusing on cytokinesis have used multinucleation as the endpoint readout. Here, we report an automated image-based detection method of intercellular bridges, which we applied to further our understanding of late cytokinetic signaling by performing an RNAi screen of ubiquitin ligases and deubiquitinases. A secondary validation confirmed four candidate genes, i.e.,

Indexed as

Nuclear ProteinsUbiquitinCell Cycle ProteinsCytokinesisHumansProtein BindingCell Cycle ProteinsCep55 protein, humanNuclear ProteinsUbiquitinCEP55cytokinesishigh-content screeningimage analysis toolRNAiubiquitin signaling

Identifiers

PMID36497121
PMCPMC9737832
OpenAlexW4311049235

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.