ArticleJournal of cell science2022
Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast.
Article in Journal of cell science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- An adaptable, self-organizing, single-cell morphology circuit optimizes suctorian predatory trap structure.Current biology : CB · 2026Article
- IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species.PLoS biology · 2026Article
- Combining ultrastructure expansion microscopy with immunofluorescence and Oligopaint DNA FISH.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Article
- An Advanced Mobile Laboratory to enable field-based microbial ecology and cell biology across scales.bioRxiv : the preprint server for biology · 2026Article
- Plants in the expansion era: recent advances in expansion microscopy in plant systems.Journal of experimental botany · 2025Review
- Dynamic remodeling of centrioles and the microtubule cytoskeleton in the lifecycle of chytrid fungi.Molecular biology of the cell · 2025Article
- A self-organizing single-cell morphology circuit optimizesbioRxiv : the preprint server for biology · 2025Article
- Root expansion microscopy: A robust method for super resolution imaging in Arabidopsis.The Plant cell · 2025Article
- Ultrastructure Expansion Microscopy (U-ExM) of the Fission Yeast Schizosaccharomyces pombe.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Nuclear pore complexes undergo Nup221 exchange during blood-stage asexual replication ofmSphere · 2024Article
- Mosquito Tissue Ultrastructure-Expansion Microscopy (MoTissU-ExM) enables ultrastructural and anatomical analysis of malaria parasites and their mosquito.BMC methods · 2024Article
- Expansion microscopy reveals characteristic ultrastructural features of pathogenic budding yeast species.Journal of cell science · 2024Article
- Spindle architecture constrains karyotype evolution.Nature cell biology · 2024Article
- Article
- Ascaris Mouse Model Protocols: Advancing Research on Larval Ascariasis Biology.Current protocols · 2024Article
- Spindle architecture constrains karyotype in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- Combining single-molecule and expansion microscopy in fission yeast to visualize protein structures at the nanostructural level.Open biology · 2024Article
- Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy.Nanoscale · 2023Article
- An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) ofBio-protocol · 2023Article
- Microtubule-mitochondrial attachment facilitates cell division symmetry and mitochondrial partitioning in fission yeast.Journal of cell science · 2023Article
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10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The budding and fission yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe have served as invaluable model organisms to study conserved fundamental cellular processes. Although super-resolution microscopy has in recent years paved the way to a better understanding of the spatial organization of molecules in cells, its wide use in yeasts has remained limited due to the specific know-how and instrumentation required, contrasted with the relative ease of endogenous tagging and live-cell fluorescence microscopy. To facilitate super-resolution microscopy in yeasts, we have extended the ultrastructure expansion microscopy (U-ExM) method to both S. cerevisiae and S. pombe, enabling a 4-fold isotropic expansion. We demonstrate that U-ExM allows imaging of the microtubule cytoskeleton and its associated spindle pole body, notably unveiling the Sfi1p-Cdc31p spatial organization on the appendage bridge structure. In S. pombe, we validate the method by monitoring the homeostatic regulation of nuclear pore complex number through the cell cycle. Combined with NHS-ester pan-labelling, which provides a global cellular context, U-ExM reveals the subcellular organization of these two yeast models and provides a powerful new method to augment the already extensive yeast toolbox. This article has an associated First Person interview with Kerstin Hinterndorfer and Felix Mikus, two of the joint first authors of the paper.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.