ArticleNature communications2022
STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Genome-wide chromatin recording resolves dynamic cell state changes.Cell systems · 2026Article
- Genome-wide chromatin recording resolves dynamic cell state changes.bioRxiv : the preprint server for biology · 2026Article
- Chromatin dynamics of the Klf4 locus in mouse pluripotent cells.Scientific reports · 2026Article
- AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications.Science advances · 2026Article
- Recent advances in methodologies of epigenomics.Epigenomics · 2026Review
- Hallmarks of glucocorticoid receptor condensates involvement in transcription regulation.iScience · 2025Article
- AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications.bioRxiv : the preprint server for biology · 2025Article
- Measurement of Spatial Contact Map Using Sequential FISH.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Image Analysis Protocol for DNA/RNA/Immunofluorescence (IF)-seqFISH Data.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Transcription-coupled changes in genomic region proximities during transcriptional bursting.Science advances · 2024Article
- Chromatin endogenous cleavage provides a global view of yeast RNA polymerase II transcription kinetics.eLife · 2024Article
- Chromatin endogenous cleavage provides a global view of yeast RNA polymerase II transcription kinetics.bioRxiv : the preprint server for biology · 2024Article
- Correlative single molecule lattice light sheet imaging reveals the dynamic relationship between nucleosomes and the local chromatin environment.Nature communications · 2024Article
- Emerging insights into transcriptional condensates.Experimental & molecular medicine · 2024Review
- Toward a comprehensive view of gene architecture during transcription.Current opinion in genetics & development · 2024Review
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Time will tell: comparing timescales to gain insight into transcriptional bursting.Trends in genetics : TIG · 2024Review
- Amphiphiles Formed from Synthetic DNA-Nanomotifs Mimic the Stepwise Dispersal of Transcriptional Clusters in the Cell Nucleus.Nano letters · 2023Article
- Live-cell imaging of chromatin contacts opens a new window into chromatin dynamics.Epigenetics & chromatin · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Transcription is a dynamic process. To detect the dynamic relationship among protein clusters of RNA polymerase II and coactivators, gene loci, and transcriptional activity, we insert an MS2 repeat, a TetO repeat, and inteins with a selection marker just downstream of the transcription start site. By optimizing the individual elements, we develop the Spliced TetO REpeAt, MS2 repeat, and INtein sandwiched reporter Gene tag (STREAMING-tag) system. Clusters of RNA polymerase II and BRD4 are observed proximal to the transcription start site of Nanog when the gene is transcribed in mouse embryonic stem cells. In contrast, clusters of MED19 and MED22 tend to be located near the transcription start site, even without transcription activity. Thus, the STREAMING-tag system reveals the spatiotemporal relationships between transcriptional activity and protein clusters near the gene. This powerful tool is useful for quantitatively understanding transcriptional regulation in living cells.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.