Evidence map›Paper›PMID 36574182›Full record

ArticleMolecular biotechnology2023

Inhibition of the Glycolysis Prevents the Cerebral Infarction Progression Through Decreasing the Lactylation Levels of LCP1.

Wei Zhang, Liang Xu, Zhenfei Yu, Meiqi Zhang, Jingquan Liu, Jianming Zhou

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.

0numbers the graph read from it
0cells of the map it votes in
55citing papers in PubMed
5.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

55 citing papers in PubMed, 68 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Wei ZhangDepartment of Critical Care Medicine, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, No.453, Tiyuchang Road, Hangzhou, 310007, Zhejiang, China.
Liang XuDepartment of Critical Care Medicine, Zhejiang Provincial People's Hospital, Hangzhou, China.
Zhenfei YuDepartment of Critical Care Medicine, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, No.453, Tiyuchang Road, Hangzhou, 310007, Zhejiang, China.
Meiqi ZhangDepartment of Critical Care Medicine, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, No.453, Tiyuchang Road, Hangzhou, 310007, Zhejiang, China.
Jingquan LiuDepartment of Critical Care Medicine, Zhejiang Provincial People's Hospital, Hangzhou, China.
Jianming ZhouDepartment of Critical Care Medicine, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, No.453, Tiyuchang Road, Hangzhou, 310007, Zhejiang, China. drzhoujianming@163.com.ORCID http://orcid.org/0000-0002-8840-7555
Zhejiang Chinese Medical University · CNZhejiang Provincial People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cerebral infarction (CI), also known as ischemic stroke, has a high incidence rate and mortality rate. The purpose of this study was to investigate the potential effect and mechanism of Lymphocyte cytosolic protein 1 (LCP1) in the CI progression. The middle cerebral artery occlusion (MCAO) treated rats and oxygen-glucose deprivation/reoxygenation (OGD/R) stimulated PC12 cells were used to establish CI model in vivo and in vitro. The cell proliferation and apoptosis was determined by CCK-8 assay and flow cytometry, respectively. Immunoprecipitation and western blot was performed to test the lactylation levels of LCP1. The cells were treated with cycloheximide to determined the protein stability of LCP1. The glucose uptake and lactate production was determined with commercial kits. The extracellular acidification rate were evaluated by Seahorse. The results showed that LCP1 was upregulated in the MCAO rats and OGD/R stimulated PC12 cells. LCP1 knockdown dramatically decreased the neurological score, infarct volume and the brain water content of MCAO rats. Besides, LCP1 knockdown promoted the cell viability while decreased the apoptosis rate of the OGD/R stimulated PC12 cells. Additionally, the global lactylation and lactylation levels of LCP1 was prominently enhanced in vivo and in vitro in cerebral infarction. 2-DG treatment prominently decreased it. In conclusion, inhibiting the glycolysis decreased the lactylation levels of LCP1 and resulted in the degradation of LCP1, which eventually relieved the CI progression.

Indexed as

Brain IschemiaInfarction, Middle Cerebral ArteryMicrofilament ProteinsAnimalsApoptosisCalcium-Binding ProteinsGlycolysisRatsCalcium-Binding Proteinslymphocyte cytosolic protein 1, ratMicrofilament ProteinsCerebral infarctionGlycolysisLactylationLCP1

Identifiers

PMID36574182
PMCPMC10352161
OpenAlexW4312211948

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.