Evidence map›Paper›PMID 36578568›Full record

ArticleFrontiers in microbiology2022

FISHing for ciliates: Catalyzed reporter deposition fluorescence

Gianna Dirren-Pitsch, Dominique Bühler, Michaela M Salcher, Barbara Bassin, Alizée Le Moigne, Martina Schuler, Jakob Pernthaler, Thomas Posch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Gianna Dirren-PitschLimnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.
Dominique BühlerLimnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.
Michaela M SalcherDepartment of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre of the Czech Academy of Sciences, České Budĕjovice, Czechia.
Barbara BassinLimnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.
Alizée Le MoigneLimnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.
Martina SchulerLimnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.
Jakob PernthalerLimnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.
Thomas PoschLimnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Planktonic ciliate species form multiple trophic guilds and are central components of freshwater food webs. Progress in molecular analytical tools has opened new insight into ciliate assemblages. However, high and variable 18S rDNA copy numbers, typical for ciliates, make reliable quantification by amplicon sequencing extremely difficult. For an exact determination of abundances, the classical morphology-based quantitative protargol staining is still the method of choice. Morphotype analyses, however, are time consuming and need specific taxonomic expertise. Catalyzed reporter deposition fluorescence

Indexed as

CARD-FISHciliate quantificationfluorescence in situ hybridizationlake water samplesplanktonic ciliatesquantitative protargol staining

Identifiers

PMID36578568
PMCPMC9790926

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.