ArticleCell proliferation2023
A chemically defined system supports two distinct types of stem cell from a single blastocyst and their self-assembly to generate blastoid.
Article in Cell proliferation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 13 citations in OpenAlex.
- From Static to Dynamic: Fluorescence Imaging Technology Advances Precise Embryo Evaluation.Small methods · 2026Review
- Article
- NELFA supports naïve pluripotency and drives 8C-like state in human embryonic stem cells.Nature communications · 2026Article
- Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation.Protein & cell · 2026Article
- The role of ASS1 in mouse embryonic stem cell differentiation into mesendoderm lineages.Stem cell research & therapy · 2025Article
- Stem cell-based embryo models: a tool to study early human development.Science China. Life sciences · 2025Review
- Defining core signaling pathways for supporting in vitro maintenance of pig extraembryonic endoderm (XEN) cells.Reproduction (Cambridge, England) · 2025Article
- Hallmarks of totipotent and pluripotent stem cell states.Cell stem cell · 2024Review
- Blastoids generated purely from embryonic stem cells both in mice and humans.Science China. Life sciences · 2024Article
- NELFA and BCL2 induce the 2C-like state in mouse embryonic stem cells in a chemically defined medium.Cell proliferation · 2024Article
- A chemically defined system supports two distinct types of stem cell from a single blastocyst and their self-assembly to generate blastoid.Cell proliferation · 2023Article
- Establishment of bovine trophoblast stem cells.Cell reports · 2023Article
- Human pluripotent-stem-cell-derived organoids for drug discovery and evaluation.Cell stem cell · 2023Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The pluripotent stem cells exist in a narrow window during early development and its derivation depends on intrinsic and extrinsic growth signalling in vitro. It has remained challenging to derive two or three distinct cell lines that are representative of blastocyst-stage lineages from one preimplantation embryo simultaneously in a chemical defined condition. Therefore, it is desirable to establish a system by manipulating extrinsic signalling in culture to derive multiple types of stem cells from a single blastocyst. This study used a defined medium containing Activin A, WNT activator and LIF (ACL medium), enabling establishment of ACL-ESCs and ACL-XEN cells from one blastocyst. ACL-blastoids were generated by suspending ACL-ESCs and ACL-XEN cells with ACL-blastoid medium in three-dimensional culture system. Lineage markers expression of ACL-blastoids were performed by immunofluorescence. Our results indicate that ACL-ESCs and ACL-XEN cells derived from one blastocyst represent ICM and PrE lineages. Importantly, we obtained ACL-blastoid from ACL-ESCs and ACL-XEN cells self-aggregation, partially recapitulating early development and initiation of early implantation events. This study would not only provide ACL culture system for derivation and maintenance of two types of cell lines corresponding to ICM as well as PrE, but also reconstruct blastoids with them to deepen our understanding of early embryogenesis and widen insights into translational application of stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.