Evidence map›Paper›PMID 36630901›Full record

ArticleStem cell reports2023

Comprehensive characterization of fetal and mature retinal cell identity to assess the fidelity of retinal organoids.

Hani Jieun Kim, Michelle O'Hara-Wright, Daniel Kim, To Ha Loi, Benjamin Y Lim, Robyn V Jamieson, Anai Gonzalez-Cordero, Pengyi Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
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  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Frontiers in cell and developmental biology · 2025
    Review
  11. DominantFrontiers in cell and developmental biology · 2025
    Article
  12. Characterization of RNA editing and gene therapy with a compact CRISPR-Cas13 in the retina.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  13. Article
  14. Toward Retinal Organoids in High-Throughput.Cold Spring Harbor perspectives in medicine · 2024
    Review
  15. Review
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hani Jieun KimComputational Systems Biology Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia; School of Mathematics and Statistics, The University of Sydney, Sydney, NSW 2006, Australia; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
Michelle O'Hara-WrightSchool of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia; Stem Cell Medicine Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia.
Daniel KimComputational Systems Biology Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
To Ha LoiSchool of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia; Eye Genetics Research Unit, Children's Medical Research Institute, Sydney Children's Hospitals Network, Save Sight Institute, The University of Sydney, Westmead, NSW 2145, Australia.
Benjamin Y LimStem Cell Medicine Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia.
Robyn V JamiesonSpecialty of Genomic Medicine, Faculty of Medicine and Health, University of Sydney, Westmead, NSW 2145, Australia; Eye Genetics Research Unit, Children's Medical Research Institute, Sydney Children's Hospitals Network, Save Sight Institute, The University of Sydney, Westmead, NSW 2145, Australia.
Anai Gonzalez-CorderoSchool of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia; Stem Cell Medicine Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia. Electronic address: agonzalez-cordero@cmri.org.au.
Pengyi YangComputational Systems Biology Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia; School of Mathematics and Statistics, The University of Sydney, Sydney, NSW 2006, Australia; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia. Electronic address: pengyi.yang@sydney.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Characterizing cell identity in complex tissues such as the human retina is essential for studying its development and disease. While retinal organoids derived from pluripotent stem cells have been widely used to model development and disease of the human retina, there is a lack of studies that have systematically evaluated the molecular and cellular fidelity of the organoids derived from various culture protocols in recapitulating their in vivo counterpart. To this end, we performed an extensive meta-atlas characterization of cellular identities of the human eye, covering a wide range of developmental stages. The resulting map uncovered previously unknown biomarkers of major retinal cell types and those associated with cell-type-specific maturation. Using our retinal-cell-identity map from the fetal and adult tissues, we systematically assessed the fidelity of the retinal organoids in mimicking the human eye, enabling us to comprehensively benchmark the current protocols for retinal organoid generation.

Indexed as

Induced Pluripotent Stem CellsPluripotent Stem CellsAdultCell DifferentiationHumansNeuronsOrganoidsRetinacell identityeyefidelityhuman eyematurationpluripotent stem cellsretinaretinal organoids

Identifiers

PMID36630901
PMCPMC9860116

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.