Evidence map›Paper›PMID 36635458›Full record

ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2023

CircZNF236 facilitates malignant progression in oral squamous cell carcinoma by sequestering miR-145-5p.

Qing Lu, Hongze Che, Yanhai Che, Min Hu

Abstract read
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In one paragraph

Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Qing LuDepartment of General Dentistry, Hospital of Stomatology, Jilin University, Changchun, China.ORCID http://orcid.org/0000-0001-9255-7743
Hongze CheSchool of Dentistry, Beihua University, Jilin, China.
Yanhai CheDepartment of Science and Education, Hospital of Stomatology, Jilin University, Changchun, China.
Min HuDepartment of Orthodontic, Hospital of Stomatology, Jilin University, 1500 Qing Hua Road, Changchun, 130021, Jilin, China. humin@jlu.edu.cn.
Jilin University · CNBeihua University · CN

Funding

Natural Science Foundation of Jilin Province YDZJ202201ZYTS251Postdoctoral Research Foundation of China 2020M670865
6 · The paper itself

Abstract

backgroundA number of non-coding circular RNAs (circRNAs) have recently been implicated in the modulation of gene expression in cancer models. We therefore sought to explore if circZNF236 has a role in oral squamous cell carcinoma (OSCC).

methodsWe first examined circZNF236 expression in 32 pairs of OSCC and noncancerous tissues. We then investigated a functional role for circZNF236 using knockdown and overexpression approaches in OSCC cancer cell lines. Cell counting kit-8, wound healing, Transwell, and flow cytometry were employed to assess circZNF236 function in vitro. The association between circZNF236 and miR-145-5p, or that between miR-145-5p and malignant brain tumor domain containing 1 (MBTD1) was predicted by bioinformatics and demonstrated by dual-luciferase reporter assays, RNA pull-down assays as well as RNA immunoprecipitation (RIP) assays. A mouse OSCC xenograft model was employed to demonstrate the impacts of circZNF236 inhibition on tumor development in vivo.

resultsOSCC tissues and cells had higher levels of circZNF236 expression compared with normal controls. Furthermore, high circZNF236 levels in patients with OSCC correlated with a poor prognosis. CircZNF236 silencing decreased the malignant properties of OSCC cells and suppressed OSCC tumor formation in the mouse model. We then noticed that miR-145-5p can be regulated by circZNF236, and that circZNF2361 promoted OSCC development by absorbing miR-145-5p and consequently upregulating MBTD1 expression.

conclusionCircZNF236 modulates OSCC via the miR-145-5p/MBTD1 axis. These results support the potential of circZNF236 as a treatment target for OSCC.

Indexed as

Carcinoma, Squamous CellHead and Neck NeoplasmsMicroRNAsMouth NeoplasmsAnimalsCell Line, TumorCell ProliferationChromosomal Proteins, Non-HistoneDisease Models, AnimalGene Expression Regulation, NeoplasticHumansMiceSquamous Cell Carcinoma of Head and NeckChromosomal Proteins, Non-HistoneMBTD1 protein, humanMicroRNAsMIRN145 microRNA, humanCircular RNAscircZNF236MBTD1miR-145-5pOral squamous cell cancer

Identifiers

PMID36635458
OpenAlexW4315778734

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.