ArticleBiophysical journal2023
Remodeling of yeast vacuole membrane lipidomes from the log (one phase) to stationary stage (two phases).
Article in Biophysical journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Mammalian MemPrep establishes the lipid composition of ER membranes in HEK293T cells.EMBO reports · 2026Article
- The yeast mitochondrial porin represses Snf1/AMP kinase signaling to attenuate viral replication.Genetics · 2026Article
- The Lack of a COPII Cargo Receptor Erv14 Impacts Physiological Functions of the Vacuole in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2026Article
- Transport of sphingolipids by yeast Npc2 supports phase separation of the vacuole membrane.The Journal of biological chemistry · 2026Article
- Coupling between sterol and sphingolipid structure in ordered membrane domains.bioRxiv : the preprint server for biology · 2026Article
- Comparative microbial lipidomics: structural diversity, adaptive strategies, and analytical advances.Folia microbiologica · 2026Review
- Transport of sphingolipids by yeast Npc2 supports phase separation of the vacuole membrane.bioRxiv : the preprint server for biology · 2025Article
- Pex30-dependent membrane contact sites maintain ER lipid homeostasis.The Journal of cell biology · 2025Article
- Temperature adaptation of yeast phospholipid molecular species at the acyl chain positional level.FEBS letters · 2025Article
- Thermotolerant yeasts promoting climate-resilient bioproduction.FEMS yeast research · 2025Review
- Sterol-lipids enable large-scale, liquid-liquid phase separation in bilayer membranes of only two components.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The ER-SURF pathway uses ER-mitochondria contact sites for protein targeting to mitochondria.EMBO reports · 2024Article
- MemPrep, a new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress.The EMBO journal · 2024Article
- Automated quantification of vacuole fusion and lipophagy inAutophagy · 2024Article
- Intracellular sphingolipid sorting drives membrane phase separation in the yeast vacuole.The Journal of biological chemistry · 2024Article
- Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells.Methods in enzymology · 2024Article
- Experimentally determined leaflet-leaflet phase diagram of an asymmetric lipid bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Upon nutrient limitation, budding yeast of Saccharomyces cerevisiae shift from fast growth (the log stage) to quiescence (the stationary stage). This shift is accompanied by liquid-liquid phase separation in the membrane of the vacuole, an endosomal organelle. Recent work indicates that the resulting micrometer-scale domains in vacuole membranes enable yeast to survive periods of stress. An outstanding question is which molecular changes might cause this membrane phase separation. Here, we conduct lipidomics of vacuole membranes in both the log and stationary stages. Isolation of pure vacuole membranes is challenging in the stationary stage, when lipid droplets are in close contact with vacuoles. Immuno-isolation has previously been shown to successfully purify log-stage vacuole membranes with high organelle specificity, but it was not previously possible to immuno-isolate stationary-stage vacuole membranes. Here, we develop Mam3 as a bait protein for vacuole immuno-isolation, and demonstrate low contamination by non-vacuolar membranes. We find that stationary-stage vacuole membranes contain surprisingly high fractions of phosphatidylcholine lipids (∼40%), roughly twice as much as log-stage membranes. Moreover, in the stationary stage, these lipids have higher melting temperatures, due to longer and more saturated acyl chains. Another surprise is that no significant change in sterol content is observed. These lipidomic changes, which are largely reflected on the whole-cell level, fit within the predominant view that phase separation in membranes requires at least three types of molecules to be present: lipids with high melting temperatures, lipids with low melting temperatures, and sterols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.