Evidence mapPaperPMID 36639741Full record

ArticleBMC ophthalmology2023

25-hydroxyvitamin D3 inhibits oxidative stress and ferroptosis in retinal microvascular endothelial cells induced by high glucose through down-regulation of miR-93.

Dongmei Zhan, Juan Zhao, Qin Shi, Juan Lou, Weiling Wang

Open access · goldAbstract read
In one paragraph

Article in BMC ophthalmology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
9.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Review
  10. Dietary Supplementation with 25-Hydroxyvitamin DAntioxidants (Basel, Switzerland) · 2024
    Article
  11. Observational
  12. Review
  13. Article
  14. Review
  15. Review
  16. Review
  17. Review
  18. Review
  19. Article
  20. Oxidative Stress and Its Regulation in Diabetic Retinopathy.Antioxidants (Basel, Switzerland) · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Dongmei ZhanDepartment of Ophthalmology, General Hospital of Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, China. zdm8620@163.com.
Juan ZhaoDepartment of Ophthalmology, General Hospital of Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, China.
Qin ShiDepartment of Ophthalmology, General Hospital of Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, China.
Juan LouDepartment of Ophthalmology, General Hospital of Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, China.
Weiling WangDepartment of Ophthalmology, General Hospital of Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, China.
Ningxia Medical University · CN

Funding

Natural Science Foundation of Ningxia, China No. 2021AAC03376
6 · The paper itself

Abstract

backgroundThe decrease of vitamin D plays a critical role in diabetes mellitus (DM)-induced oxidative stress and vascular endothelial injury. Therefore, we investigated the effect and mechanism of 25-hydroxyvitamin D3 (25 (OH) D3) on oxidative stress and ferroptosis induced by high glucose in human retinal microvascular endothelial cells (hRMVECs). And the objective of this paper was to propose a new strategy for the prevention and treatment of diabetic retinopathy (DR).

methodsFirst, hRMVECs were transfected with mimics NC or miR-93. After that, cells were treated with 100 nM / 500 nM 25 (OH) D3 and then cultured in a high glucose (30 mM) environment. Subsequently, qRT-PCR was employed to detect the expression level of miR-93; CCK-8 for the proliferation of cells in each group; biochemical tests for the level of intracellular reactive oxygen species (ROS), malondialdehyde (MDA), reduced glutathione (GSH) and ferrous ion (Fe

resultsUnder a high glucose environment, 25 (OH) D3 at 100 nM/500 nM could significantly promote the proliferation of hRMVECs, remarkably decrease the level of intracellular ROS/MDA, and up-regulate the level of GSH. Besides, 25 (OH) D3 greatly reduced Fe

conclusion25 (OH) D3 may inhibit oxidative stress and ferroptosis in hRMVECs induced by high glucose via down-regulation of miR-93.

Indexed as

3,4-MethylenedioxyamphetamineFerroptosisMicroRNAsCalcifediolDown-RegulationEndothelial CellsGlucoseHumansHyperaldosteronismOxidative StressReactive Oxygen Species3,4-MethylenedioxyamphetamineCalcifediolGlucoseMicroRNAsMIRN93 microRNA, humanReactive Oxygen Species25-hydroxyvitamin D3(25 (OH) D3)FerroptosisHuman retinal microvascular endothelial cells (hRMVECs)MiR-93Oxidative stress

Identifiers

PMID36639741
PMCPMC9840274
OpenAlexW4315874481

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.