Evidence map›Paper›PMID 36657883›Full record

ArticleAnalytica chimica acta2023

Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70): Application in diagnosis/prognosis of coronavirus disease 2019 (COVID-19).

Masoud Negahdary, Mario Hiroyuki Hirata, Solange Kazumi Sakata, Rozana Mesquita Ciconelli, Gisele Medeiros Bastos, Jéssica Bassani Borges, Helena Strelow Thurow, Alceu Totti Silveira Junior, Marcelo Ferraz Sampaio, Larissa Berretta Guimarães and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytica chimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Masoud NegahdaryDepartment of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, 05508-000, São Paulo, Brazil. Electronic address: mnegahdary@iq.usp.br.
Mario Hiroyuki HirataDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo, Av Prof Lineu Prestes 580, 05508-000, São Paulo, Brazil.
Solange Kazumi SakataNuclear and Energy Research Institute, National Commission of Nuclear Energy (IPEN/CNEN - SP), São Paulo, SP, 05508-000, Brazil.
Rozana Mesquita CiconelliResearch and Education Division, Hospital A Beneficência Portuguesa de São Paulo, São Paulo, Brazil.
Gisele Medeiros BastosResearch and Education Division, Hospital A Beneficência Portuguesa de São Paulo, São Paulo, Brazil.
Jéssica Bassani BorgesResearch and Education Division, Hospital A Beneficência Portuguesa de São Paulo, São Paulo, Brazil.
Helena Strelow ThurowResearch and Education Division, Hospital A Beneficência Portuguesa de São Paulo, São Paulo, Brazil.
Alceu Totti Silveira JuniorDepartment of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, 05508-000, São Paulo, Brazil.
Marcelo Ferraz SampaioResearch and Education Division, Hospital A Beneficência Portuguesa de São Paulo, São Paulo, Brazil.
Larissa Berretta GuimarãesResearch and Education Division, Hospital A Beneficência Portuguesa de São Paulo, São Paulo, Brazil.
Bruno Sussumu MaedaResearch and Education Division, Hospital A Beneficência Portuguesa de São Paulo, São Paulo, Brazil.
Lúcio AngnesDepartment of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, 05508-000, São Paulo, Brazil. Electronic address: luangnes@iq.usp.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this research, by using aptamer-conjugated gold nanoparticles (aptamer-AuNPs) and a modified glassy carbon electrode (GCE) with reduced graphene oxide (rGO) and Acropora-like gold (ALG) nanostructure, a sandwich-like system provided for sensitive detection of heat shock protein 70 kDa (HSP70), which applied as a functional biomarker in diagnosis/prognosis of COVID-19. Initially, the surface of the GCE was improved with rGO and ALG nanostructures, respectively. Then, an aptamer sequence as the first part of the bioreceptor was covalently bound on the surface of the GCE/rGO/ALG nanostructures. After adding the analyte, the second part of the bioreceptor (aptamer-AuNPs) was immobilized on the electrode surface to improve the diagnostic performance. The designed aptasensor detected HSP70 in a wide linear range, from 5 pg mL

Indexed as

Aptamers, NucleotideBiosensing TechniquesCOVID-19GraphiteMetal NanoparticlesCarbonCOVID-19 TestingElectrochemical TechniquesElectrodesGoldHumansLimit of DetectionPrognosisAptamers, NucleotideCarbonGoldgraphene oxideGraphiteAptasensorBioelectrochemistryCoronavirus disease 2019 (COVID-19)Gold nanomaterialsHeat shock protein 70 kDa (HSP70)Reduced graphene oxide (rGO)

Identifiers

PMID36657883
PMCPMC9729787

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.