ArticleThe Plant cell2023
Mapping the signaling network of BIN2 kinase using TurboID-mediated biotin labeling and phosphoproteomics.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 88 citations in OpenAlex.
- Best practices for biotin ligase-based proximity labeling proteomics in plant systems.The Plant cell · 2026Review
- Brassinosteroids promote sugar synthesis by inhibiting BIN2 phosphorylation of phosphoenolpyruvate carboxykinase.Nature plants · 2026Article
- Brassinosteroids boost sugar production by inhibiting PCK phosphorylation.Nature plants · 2026Article
- Application of Proximity-Labeling Techniques in Plants: A Review of Successful Cases.International journal of molecular sciences · 2026Review
- DELLA proteins interact with HAC1 and control plant growth via transcriptional regulation of the growth repressor gene ERF2.The Plant cell · 2026Article
- Linking sugar sensing to immunity in plants through O-glycosylation of immune-signaling kinases.bioRxiv : the preprint server for biology · 2026Article
- A BIN2-SOG1 molecular module regulates replication stress response independently on ATR.Science advances · 2026Article
- CabioRxiv : the preprint server for biology · 2026Article
- Masters of perception: phosphorylation-dependent signaling in plants.The New phytologist · 2026Review
- Arabidopsis microtubule-BRI1-associated proteins negatively regulate hypocotyl elongation by controlling brassinosteroid-dependent cortical microtubule reorientation.Plant communications · 2026Article
- The study of plasmodesmal biology using proximity labeling technologies.Journal of experimental botany · 2026Review
- Integrating endogenous TurboID and data-independent acquisition mass spectrometry for in vivo proximity labeling.The EMBO journal · 2026Article
- Protein Post-Translational Modifications in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2025Review
- NEDD4 suppresses ferroptosis in lung ischemia-reperfusion injury by ubiquitinating and degrading SLC1A5.Scientific reports · 2025Article
- The Ste20 Homologue FvM4K1 Regulates Organ Size via Hippo Signalling Pathway in Woodland Strawberry (Fragaria vesca).Plant biotechnology journal · 2025Article
- Specificity of brassinosteroid perception and its integration at signaling crossroads in vascular development.The Plant journal : for cell and molecular biology · 2025Review
- Brassinosteroids promote sugar synthesis by inhibiting BIN2 phosphorylation of phosphoenolpyruvate carboxykinase.bioRxiv : the preprint server for biology · 2025Article
- Natural variation in the GhTTL promoter modulates cotton fiber elongation through the GhTALE-GhTTL-GhBIN2 module.Plant communications · 2025Article
- Biotinylation Interferes with Protein Ubiquitylation and Turnover in Arabidopsis-A Cautionary Insight for Proximity Labeling in Ubiquitylation Proteome Studies.International journal of molecular sciences · 2025Article
- Split-YFP-coupled interaction-dependent TurboID identifies new functions of basal cell polarity inProceedings of the National Academy of Sciences of the United States of America · 2025Article
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18 authors at 6 institutions in 4 countries.
Funding
Abstract
Elucidating enzyme-substrate relationships in posttranslational modification (PTM) networks is crucial for understanding signal transduction pathways but is technically difficult because enzyme-substrate interactions tend to be transient. Here, we demonstrate that TurboID-based proximity labeling (TbPL) effectively and specifically captures the substrates of kinases and phosphatases. TbPL-mass spectrometry (TbPL-MS) identified over 400 proximal proteins of Arabidopsis thaliana BRASSINOSTEROID-INSENSITIVE2 (BIN2), a member of the GLYCOGEN SYNTHASE KINASE 3 (GSK3) family that integrates signaling pathways controlling diverse developmental and acclimation processes. A large portion of the BIN2-proximal proteins showed BIN2-dependent phosphorylation in vivo or in vitro, suggesting that these are BIN2 substrates. Protein-protein interaction network analysis showed that the BIN2-proximal proteins include interactors of BIN2 substrates, revealing a high level of interactions among the BIN2-proximal proteins. Our proteomic analysis establishes the BIN2 signaling network and uncovers BIN2 functions in regulating key cellular processes such as transcription, RNA processing, translation initiation, vesicle trafficking, and cytoskeleton organization. We further discovered significant overlap between the GSK3 phosphorylome and the O-GlcNAcylome, suggesting an evolutionarily ancient relationship between GSK3 and the nutrient-sensing O-glycosylation pathway. Our work presents a powerful method for mapping PTM networks, a large dataset of GSK3 kinase substrates, and important insights into the signaling network that controls key cellular functions underlying plant growth and acclimation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.