Evidence map›Paper›PMID 36671885›Full record

ReviewBiosensors2022

Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications.

Alessio Meggiolaro, Valentina Moccia, Paola Brun, Matteo Pierno, Giampaolo Mistura, Valentina Zappulli, Davide Ferraro

Open access · goldAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
4.3field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 60 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Alessio MeggiolaroDepartment of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy.ORCID 0000-0002-2844-0767
Valentina MocciaDepartment of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, 35020 Legnaro, Italy.ORCID 0000-0001-8843-0395
Paola BrunDepartment of Molecular Medicine, University of Padua, Via Gabelli 63, 35121 Padua, Italy.ORCID 0000-0002-3279-9000
Matteo PiernoDepartment of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy.ORCID 0000-0002-8973-0338
Giampaolo MisturaDepartment of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy.ORCID 0000-0002-3426-5475
Valentina ZappulliDepartment of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, 35020 Legnaro, Italy.ORCID 0000-0001-6203-0190
Davide FerraroDepartment of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy.ORCID 0000-0002-6760-7988
University of Padua · IT

Funding

University of Padua BIRD 2021 - BiodivseqUniversity of Padua STARS 2019
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are double-layered lipid membrane vesicles released by cells. Currently, EVs are attracting a lot of attention in the biological and medical fields due to their role as natural carriers of proteins, lipids, and nucleic acids. Thus, they can transport useful genomic information from their parental cell through body fluids, promoting cell-to-cell communication even between different organs. Due to their functionality as cargo carriers and their protein expression, they can play an important role as possible diagnostic and prognostic biomarkers in various types of diseases, e.g., cancers, neurodegenerative, and autoimmune diseases. Today, given the invaluable importance of EVs, there are some pivotal challenges to overcome in terms of their isolation. Conventional methods have some limitations: they are influenced by the starting sample, might present low throughput and low purity, and sometimes a lack of reproducibility, being operator dependent. During the past few years, several microfluidic approaches have been proposed to address these issues. In this review, we summarize the most important microfluidic-based devices for EV isolation, highlighting their advantages and disadvantages compared to existing technology, as well as the current state of the art from the perspective of the use of these devices in clinical applications.

Indexed as

Extracellular VesiclesNucleic AcidsCell CommunicationMicrofluidicsReproducibility of ResultsNucleic Acidsclinicsextracellular vesiclesliquid biopsymicrofabricationmicrofluidicspurification

Identifiers

PMID36671885
PMCPMC9855931
OpenAlexW4313409676

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.