Evidence map›Paper›PMID 36674549›Full record

ArticleInternational journal of molecular sciences2023

Phosphatidylethanolamines Are Associated with Nonalcoholic Fatty Liver Disease (NAFLD) in Obese Adults and Induce Liver Cell Metabolic Perturbations and Hepatic Stellate Cell Activation.

Samaa Shama, Hyejeong Jang, Xiaokun Wang, Yang Zhang, Nancy Nabil Shahin, Tarek Kamal Motawi, Seongho Kim, Samer Gawrieh, Wanqing Liu

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 35 citations in OpenAlex.

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  8. Effects of fasting and inflammatory challenges on the swine hepatic metabolome.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Samaa ShamaDepartment of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA.
Hyejeong JangBiostatistics and Bioinformatics Core, Department of Oncology, Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA.
Xiaokun WangDepartment of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA.
Yang ZhangDepartment of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA.
Nancy Nabil ShahinDepartment of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.ORCID 0000-0002-9746-5335
Tarek Kamal MotawiDepartment of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
Seongho KimBiostatistics and Bioinformatics Core, Department of Oncology, Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA.ORCID 0000-0003-1120-073X
Samer GawriehDivision of Gastroenterology and Hepatology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Wanqing LiuDepartment of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA.
Eugene Applebaum College of Pharmacy and Health Sciences · USCairo University · EGWayne State University · USIndiana University School of Medicine

Funding

Fatty Acid Desaturase 1 (FADS1) Variants and Non-alcoholic Fatty Liver DiseaseR01DK106540 · NIDDK · WAYNE STATE UNIVERSITY · PI LIU, WANQING · 2016 to 2020
$1.9M
A Precision Targeted Therapeutics for Nonalcoholic Fatty Liver DiseaseR01DK124612 · NIDDK · WAYNE STATE UNIVERSITY · PI LIU, WANQING · 2021 to 2024
$1.7M
NIDDK NIH HHS R01 DK106540NIDDK NIH HHS R01 DK124612NIH/NCI P30 CA022453 (SK)NIH/NIDDK R01DK106540 (WL)NIH/NIDDK R01DK124612 (WL)NIH/NIGMS R21GM140352 (SK)
6 · The paper itself

Abstract

Pathogenesis roles of phospholipids (PLs) in nonalcoholic fatty liver disease (NAFLD) remain incompletely understood. This study investigated the role of PLs in the progression of NAFLD among obese individuals via studying the alterations in serum PL composition throughout the spectrum of disease progression and evaluating the effects of specific phosphatidylethanolamines (PEs) on FLD development in vitro. A total of 203 obese subjects, who were undergoing bariatric surgery, were included in this study. They were histologically classified into 80 controls (C) with normal liver histology, 93 patients with simple hepatic steatosis (SS), 16 with borderline nonalcoholic steatohepatitis (B-NASH) and 14 with progressive NASH (NASH). Serum PLs were profiled by automated electrospray ionization tandem mass spectrometry (ESI-MS/MS). HepG2 (hepatoma cells) and LX2 (immortalized hepatic stellate cells or HSCs) were used to explore the roles of PL in NAFLD/NASH development. Several PLs and their relative ratios were significantly associated with NAFLD progression, especially those involving PE. Incubation of HepG2 cells with two phosphatidylethanolamines (PEs), PE (34:1) and PE (36:2), resulted in significant inhibition of cell proliferation, reduction of mitochondrial mass and membrane potential, induction of lipid accumulation and mitochondrial ROS production. Meanwhile, treatment of LX2 cells with both PEs markedly increased cell activation and migration. These effects were associated with a significant change in the expression levels of genes involved in lipogenesis, lipid oxidation, autophagy, apoptosis, inflammation, and fibrosis. Thus, our study demonstrated that elevated level of PEs increases susceptibility to the disease progression of obesity associated NAFLD, likely through a causal cascade of impacts on the function of different liver cells.

Indexed as

Non-alcoholic Fatty Liver DiseaseAdultDisease ProgressionHepatic Stellate CellsHumansLiverObesityPhosphatidylethanolaminesTandem Mass SpectrometryPhosphatidylethanolaminescell migrationfibrosismitochondrianon-alcoholic fatty liver diseaseobesityphosphatidylethanolaminephospholipids

Identifiers

PMID36674549
PMCPMC9861886
OpenAlexW4313558770

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.