Evidence map›Paper›PMID 36674576›Full record

ArticleInternational journal of molecular sciences2023

Opioid-Modulated Receptor Localization and Erk1/2 Phosphorylation in Cells Coexpressing μ-Opioid and Nociceptin Receptors.

Guan-Yu Zhuo, Ming-Chi Chen, Tzu-Yu Lin, Shih-Ting Lin, Daniel Tzu-Li Chen, Cynthia Wei-Sheng Lee

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Guan-Yu ZhuoInstitute of Translational Medicine and New Drug Development, China Medical University, Taichung 40402, Taiwan.ORCID 0000-0002-9813-2989
Ming-Chi ChenInstitute of Translational Medicine and New Drug Development, China Medical University, Taichung 40402, Taiwan.
Tzu-Yu LinCenter for Drug Abuse and Addiction, China Medical University Hospital, Taichung 40447, Taiwan.
Shih-Ting LinIntegrative Stem Cell Center, China Medical University Hospital, Taichung 40447, Taiwan.
Daniel Tzu-Li ChenSchool of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.ORCID 0000-0002-3254-5983
Cynthia Wei-Sheng LeeCenter for Drug Abuse and Addiction, China Medical University Hospital, Taichung 40447, Taiwan.ORCID 0000-0002-9783-8031
China Medical University · TW

Funding

China Medical University Hospital, Taichung, Taiwan DMR-109-138, DMR-110-055, and DMR-111-060Ministry of Science and Technology, Taiwan MOST 103-2320-B-039-049Ministry of Science and Technology, Taiwan MOST 105-2320-B-039-008Ministry of Science and Technology, Taiwan MOST 107-2320-B-039-046Ministry of Science and Technology, Taiwan MOST 110-2112-M-039-001-MY3
6 · The paper itself

Abstract

We attempted to examine the alterations elicited by opioids via coexpressed μ-opioid (MOP) and nociceptin/orphanin FQ (NOP) receptors for receptor localization and Erk1/2 (p44/42 MAPK) in human embryonic kidney (HEK) 293 cells. Through two-photon microscopy, the proximity of MOP and NOP receptors was verified by fluorescence resonance energy transfer (FRET), and morphine but not buprenorphine facilitated the process of MOP-NOP heterodimerization. Single-particle tracking (SPT) further revealed that morphine or buprenorphine hindered the movement of the MOP-NOP heterodimers. After exposure to morphine or buprenorphine, receptor localization on lipid rafts was detected by immunocytochemistry, and phosphorylation of Erk1/2 was determined by immunoblotting in HEK 293 cells expressing MOP, NOP, or MOP+NOP receptors. Colocalization of MOP and NOP on lipid rafts was enhanced by morphine but not buprenorphine. Morphine stimulated the phosphorylation of Erk1/2 with a similar potency in HEK 293 cells expressing MOP and MOP+NOP receptors, but buprenorphine appeared to activate Erk1/2 solely through NOP receptors. Our results suggest that opioids can fine-tune the cellular localization of opioid receptors and phosphorylation of Erk1/2 in MOP+NOP-expressing cells.

Indexed as

BuprenorphineReceptors, OpioidAnalgesics, OpioidHEK293 CellsHumansMorphineNociceptin ReceptorPhosphorylationReceptors, Opioid, muAnalgesics, OpioidBuprenorphineMorphineNociceptin ReceptorReceptors, OpioidReceptors, Opioid, muErk1/2 (p44/p42 MAPK)fluorescence resonance energy transfer (FRET)lipid raftsnociceptin/orphanin FQ (NOP) receptorsingle-particle tracking (SPT)two-photon microscopyμ-opioid (MOP) receptor

Identifiers

PMID36674576
PMCPMC9865058
OpenAlexW4313640527

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.