Evidence map›Paper›PMID 36698146›Full record

ArticleJournal of translational medicine2023

A multi-omic analysis reveals the esophageal dysbiosis as the predominant trait of eosinophilic esophagitis.

Luca Massimino, Alberto Barchi, Francesco Vito Mandarino, Salvatore Spanò, Luigi Antonio Lamparelli, Edoardo Vespa, Sandro Passaretti, Laurent Peyrin-Biroulet, Edoardo Vincenzo Savarino, Vipul Jairath and 2 more

Open access · goldAbstract read
In one paragraph

Article in Journal of translational medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
12.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. The Microbiome and Esophageal Disease: Where Are We Now?Current gastroenterology reports · 2026
    Review
  4. Review
  5. Review
  6. Observational
  7. Article
  8. The Dynamic Evolution of Eosinophilic Esophagitis.Diagnostics (Basel, Switzerland) · 2025
    Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Focus on Achalasia in the Omics Era.International journal of molecular sciences · 2024
    Review
  14. Review
  15. Review
  16. Development and dysfunction of structural cells in eosinophilic esophagitis.The Journal of allergy and clinical immunology · 2024
    Review
  17. Review
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 3 countries.

Luca MassiminoDepartment of Gastroenterology and Digestive Endoscopy, IRCCS Ospedale San Raffaele, Milan, Italy.
Alberto BarchiDepartment of Gastroenterology and Digestive Endoscopy, IRCCS Ospedale San Raffaele, Milan, Italy.
Francesco Vito MandarinoDepartment of Gastroenterology and Digestive Endoscopy, IRCCS Ospedale San Raffaele, Milan, Italy.
Salvatore SpanòDepartment of Gastroenterology and Digestive Endoscopy, IRCCS Ospedale San Raffaele, Milan, Italy.
Luigi Antonio LamparelliIBD Center, IRCCS Humanitas Research Hospital, Milan, Italy.
Edoardo VespaDepartment of Gastroenterology and Digestive Endoscopy, IRCCS Ospedale San Raffaele, Milan, Italy.
Sandro PassarettiDepartment of Gastroenterology and Digestive Endoscopy, IRCCS Ospedale San Raffaele, Milan, Italy.
Laurent Peyrin-BirouletInserm NGERE, University of Lorraine, Vandoeuvre-les-Nancy, France.
Edoardo Vincenzo SavarinoDepartment of Surgery, Oncology, and Gastroenterology, University of Padua, Padua, Italy.
Vipul JairathDepartment of Medicine, Division of Gastroenterology, Western University, London, ON, Canada.
Federica Ungaro *Department of Gastroenterology and Digestive Endoscopy, IRCCS Ospedale San Raffaele, Milan, Italy. ungaro.federica@hsr.it.ORCID 0000-0001-5395-7795
Silvio Danese *Department of Gastroenterology and Digestive Endoscopy, IRCCS Ospedale San Raffaele, Milan, Italy. danese.silvio@hsr.it.
IRCCS Ospedale San Raffaele · ITVita-Salute San Raffaele University · ITInserm · FRIRCCS Humanitas Research Hospital · ITUniversity of Padua · ITWestern University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEosinophilic esophagitis (EoE) is a chronic immune-mediated rare disease, characterized by esophageal dysfunctions. It is likely to be primarily activated by food antigens and is classified as a chronic disease for most patients. Therefore, a deeper understanding of the pathogenetic mechanisms underlying EoE is needed to implement and improve therapeutic lines of intervention and ameliorate overall patient wellness.

methodsRNA-seq data of 18 different studies on EoE, downloaded from NCBI GEO with faster-qdump ( https://github.com/ncbi/sra-tools ), were batch-corrected and analyzed for transcriptomics and metatranscriptomics profiling as well as biological process functional enrichment. The EoE TaMMA web app was designed with plotly and dash. Tabula Sapiens raw data were downloaded from the UCSC Cell Browser. Esophageal single-cell raw data analysis was performed within the Automated Single-cell Analysis Pipeline. Single-cell data-driven bulk RNA-seq data deconvolution was performed with MuSiC and CIBERSORTx. Multi-omics integration was performed with MOFA.

resultsThe EoE TaMMA framework pointed out disease-specific molecular signatures, confirming its reliability in reanalyzing transcriptomic data, and providing new EoE-specific molecular markers including CXCL14, distinguishing EoE from gastroesophageal reflux disorder. EoE TaMMA also revealed microbiota dysbiosis as a predominant characteristic of EoE pathogenesis. Finally, the multi-omics analysis highlighted the presence of defined classes of microbial entities in subsets of patients that may participate in inducing the antigen-mediated response typical of EoE pathogenesis.

conclusionsOur study showed that the complex EoE molecular network may be unraveled through advanced bioinformatics, integrating different components of the disease process into an omics-based network approach. This may implement EoE management and treatment in the coming years.

Indexed as

Eosinophilic EsophagitisAllergensDysbiosisHumansMultiomicsReproducibility of ResultsAllergensEsophagusMicrobiotaTranscriptomicsWeb app

Identifiers

PMID36698146
PMCPMC9875471
OpenAlexW4318045005

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.