Evidence map›Paper›PMID 36713396›Full record

ArticleFrontiers in immunology2022

Weight cycling induces innate immune memory in adipose tissue macrophages.

Heather L Caslin, Matthew A Cottam, Jacqueline M Piñon, Likem Y Boney, Alyssa H Hasty

Open access · goldAbstract read
In one paragraph

Article in Frontiers in immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
8.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 53 citations in OpenAlex.

  1. Article
  2. Article
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  4. Article
  5. T cell dysregulation and remodeling in pediatric obesity and weight loss.bioRxiv : the preprint server for biology · 2026
    Article
  6. Review
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  11. Article
  12. Review
  13. Article
  14. Weight Trajectory Impacts Risk for 10 Distinct Cardiometabolic Diseases.The Journal of clinical endocrinology and metabolism · 2025
    Article
  15. Observational
  16. Influence of Weight Loss and Weight Regain on Adipose Tissue Inflammation.Arteriosclerosis, thrombosis, and vascular biology · 2025
    Review
  17. Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Heather L CaslinDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, United States.
Matthew A CottamDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, United States.
Jacqueline M PiñonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, United States.
Likem Y BoneyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, United States.
Alyssa H HastyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, United States.
Vanderbilt University · US

Funding

Translational Analysis CoreP30DK058404 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI RICHARD M. PEEK · 2002 to 2026
$29.9M
MULTIDISCIPLINARY TRAINING IN MOLECULAR ENDOCRINOLOGYT32DK007563 · NIDDK · VANDERBILT UNIVERSITY · PI Richard M O'Brien · 1988 to 2026
$15.9M
XFe96 AnalyzerS10OD018015 · OD · VANDERBILT UNIVERSITY · PI LANNIGAN, DEBORAH · 2014 to 2014
$200k
The role of T cells in adipose tissue inflammation and insulin resistance during weight cyclingF31DK123881 · NIDDK · VANDERBILT UNIVERSITY · PI COTTAM, MATTHEW ALEXANDER · 2020 to 2022
$73k
BLRD VA I01 BX002195BLRD VA IK6 BX005649NIDDK NIH HHS F31 DK123881NIDDK NIH HHS T32 DK007563NIH HHS S10 OD018015
6 · The paper itself

Abstract

Introduction: Weight loss improves obesity-associated diabetes risk. However, most individuals regain weight, which worsens the risk of developing diabetes and cardiovascular disease. We previously reported that male mice retain obesity-associated immunological changes even after weight loss, suggesting that immune cells may remember the state of obesity. Therefore, we hypothesized that cycles of weight gain and loss, otherwise known as weight cycling, can induce innate memory in adipose macrophages. Methods: Bone marrow derived macrophages were primed with palmitic acid or adipose tissue conditioned media in a culture model of innate immune memory. Mice also put on low fat or high fat diets over 14-27 weeks to induce weight gain, weight loss, and weight cycling. Results: Priming cells with palmitic acid or adipose tissue conditioned media from obese mice increased maximal glycolysis and oxidative phosphorylation and increased LPS-induced TNFα and IL-6 production. Palmitic acid effects were dependent on TLR4 and impaired by methyltransferase inhibition and AMPK activation. While weight loss improved glucose tolerance in mice, adipose macrophages were primed for greater activation to subsequent stimulation by LPS Discussion: Together, these data suggest that weight loss following obesity can prime adipose macrophages for enhanced inflammation upon weight regain. This innate immune memory response may contribute to worsened glucose tolerance following weight cycling.

Indexed as

Diabetes MellitusInsulin ResistanceAdipose TissueAnimalsCulture Media, ConditionedGlucoseLipopolysaccharidesMacrophagesMaleMiceObesityPalmitic AcidTrained ImmunityTumor Necrosis Factor-alphaWeight CyclingWeight GainCulture Media, ConditionedGlucoseLipopolysaccharidesPalmitic AcidTumor Necrosis Factor-alphaadipose tissue macrophagesinnate immune memoryobesitytrained innate immunityweight cyclingweight loss

Identifiers

PMID36713396
PMCPMC9876596
OpenAlexW4315648741

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.