Evidence map›Paper›PMID 36717804›Full record

ArticleBMC pulmonary medicine2023

Comparative transcription profiling of mRNA and lncRNA in pulmonary arterial hypertension after C75 treatment.

Cuilan Hou, Lijian Xie, Tingxia Wang, Junmin Zheng, Yuqi Zhao, Qingzhu Qiu, Yi Yang, Tingting Xiao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC pulmonary medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Cuilan HouDepartment of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai, 200062, China.
Lijian XieDepartment of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai, 200062, China.
Tingxia WangDepartment of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai, 200062, China.
Junmin ZhengDepartment of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai, 200062, China.
Yuqi ZhaoThe First Affiliated Hospital of Anhui Medical University, No. 218 Ji-Xi Road, Hefei, 230022, Anhui, China.
Qingzhu QiuDepartment of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai, 200062, China.
Yi YangThe Children's Hospital, Zhejiang University School of Medicine, No. 3333 Binsheng Road, Binjiang District, Hangzhou, 310052, China.
Tingting XiaoDepartment of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai, 200062, China. ttxiao2017@163.com.
Shanghai Children's Hospital · CNAnhui Medical University · CNChildren's Hospital of Zhejiang University · CNJinshan Hospital of Fudan University · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo investigate mRNA and long non-coding RNA (lncRNA) expression profiles in monocrotaline (MCT)- mice. MATERIALS AND

methodsLung tissues (Control-Vehicle, MCT-Vehicle, and MCT-C75) were examined by high-throughput sequencing (HTS). Aberrantly expressed mRNAs and lncRNAs were analyzed by bioinformatics. Cell proliferation and cell cycle analysis were performed to detect the potential protective effects of C75, an inhibitor of fatty acid synthase. The signaling pathways associated with inflammatory responses were verified by real time-PCR.

resultsRNA sequencing data reveals 285 differentially expressed genes (DEGs) and 147 lncRNAs in the MCT-Vehicle group compared to the control. After five-week of C75 treatment, 514 DEGs and 84 lncRNAs are aberrant compared to the MCT-Vehicle group. Analysis of DEGs and lncRNA target genes reveals that they were enriched in pathways related to cell cycle, cell division, and vascular smooth muscle contraction that contributes to the PAH pathological process. Subsequently, the expression of eight DEGs and three lncRNAs is verified using RT-PCR. Differentially expressed lncRNAs (ENSMUSG00000110393.2, Gm38850, ENSMUSG00000100465.1, ENSMUSG00000110399.1) may associate in PAH pathogenesis as suggested by co-expression network analysis. C75 can protect against MCT-induced PAH through its anti-inflammatory and anti-proliferation.

conclusionsThese DEGs and lncRNAs can be considered as novel candidate regulators of PAH pathogenesis. We propose that C75 treatment can partially reverse PAH pathogenesis through modulating cell cycle, cell proliferation, and anti-inflammatory.

Indexed as

Hypertension, PulmonaryPulmonary Arterial HypertensionRNA, Long NoncodingAnimalsAnti-Inflammatory AgentsFamilial Primary Pulmonary HypertensionMiceRNA, MessengerAnti-Inflammatory AgentsRNA, Long NoncodingRNA, MessengerBioinformatic analysesC75Cell cycleHigh-throughput sequencingInflammatoryPulmonary arterial hypertension

Identifiers

PMID36717804
PMCPMC9887911
OpenAlexW4318669347

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.