Evidence map›Paper›PMID 36719771›Full record

ArticleHuman gene therapy2023

Direct Comparison of Epifluorescence and Immunostaining for Assessing Viral Mediated Gene Expression in the Primate Brain.

Tierney B Daw, Hala G El-Nahal, Michele A Basso, Elizabeth J Jun, Alex R Bautista, R Jude Samulski, Marc A Sommer, Martin O Bohlen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Human gene therapy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Tierney B DawDepartment of Neurobiology, Duke University School of Medicine, Durham, North Carolina, USA.
Hala G El-NahalDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Michele A BassoFuster Laboratory of Cognitive Neuroscience, Department of Psychiatry and Biobehavioral Sciences, Jane and Terry Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, Los Angeles, California, USA.
Elizabeth J JunFuster Laboratory of Cognitive Neuroscience, Department of Psychiatry and Biobehavioral Sciences, Jane and Terry Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, Los Angeles, California, USA.
Alex R BautistaFuster Laboratory of Cognitive Neuroscience, Department of Psychiatry and Biobehavioral Sciences, Jane and Terry Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, Los Angeles, California, USA.ORCID 0000-0003-4218-7953
R Jude SamulskiGene Therapy Center and Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.ORCID 0000-0001-7299-9608
Marc A SommerDepartment of Neurobiology, Duke University School of Medicine, Durham, North Carolina, USA.
Martin O BohlenDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.ORCID 0000-0001-6522-146X
Duke University · USNeurobehavioral Systems · USUniversity of North Carolina at Chapel Hill · USUniversity of Washington · US

Funding

Changes across the lifespan in the use of heuristics to guide decision-makingR01AG063090 · NIA · UNIVERSITY OF WASHINGTON · PI BASSO, MICHELE A · 2019 to 2023
$3.8M
An Open Source, Wireless, Miniature Microscope for Monitoring Neuronal ActivityUF1NS107668 · NINDS · UNIVERSITY OF WASHINGTON · PI BASSO, MICHELE A, GOLSHANI, PEYMAN · 2018 to 2018
$3.7M
Enhancement, mapping, and validation of viral vectors for primate optogeneticsR01NS125843 · NINDS · DUKE UNIVERSITY · PI Marc A Sommer · 2022 to 2026
$3.2M
Neuromuscular Control of Primate Eye MovementsR21EY030278 · NEI · DUKE UNIVERSITY · PI SOMMER, MARC A · 2019 to 2020
$429k
NEI NIH HHS R21 EY030278NIA NIH HHS R01 AG063090NINDS NIH HHS R01 NS125843NINDS NIH HHS UF1 NS107668
6 · The paper itself

Abstract

Viral vector technologies are commonly used in neuroscience research to understand and manipulate neural circuits, but successful applications of these technologies in non-human primate models have been inconsistent. An essential component to improve these technologies is an impartial and accurate assessment of the effectiveness of different viral constructs in the primate brain. We tested a diverse array of viral vectors delivered to the brain and extraocular muscles of macaques and compared three methods for histological assessment of viral-mediated fluorescent transgene expression: epifluorescence (Epi), immunofluorescence (IF), and immunohistochemistry (IHC). Importantly, IF and IHC identified a greater number of transduced neurons compared to Epi. Furthermore, IF and IHC reliably provided enhanced visualization of transgene in most cellular compartments (

Indexed as

BrainPrimatesAnimalsGene ExpressionGenetic VectorsNeuronsTransgenesimmunofluorescenceimmunohistochemistryneuronal actuatornonhuman primatetransgeneviral vector

Identifiers

PMID36719771
PMCPMC10031143
OpenAlexW4318670885

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.