ArticleProceedings of the National Academy of Sciences of the United States of America2023
Tubuloid culture enables long-term expansion of functional human kidney tubule epithelium from iPSC-derived organoids.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Single-cell transcriptomic comparison of tubular segment maturation in advanced humaniScience · 2026Article
- Organoids: From Bench to Bedside Applications.MedComm · 2026Review
- Microenvironment optimization enables kidney organoid longevity with epithelial-endothelial joint basement membrane formation.bioRxiv : the preprint server for biology · 2026Article
- Kidney Organoids in Drug Development: Integrating Technological Advances and Standardization for Effective Implementation.Advanced healthcare materials · 2026Review
- Translational Potential: Kidney Tubuloids in Precision Medicine and Regenerative Nephrology.Pharmaceutics · 2026Article
- Multiscale imaging of polarity in bovine oviductal organoids.Frontiers in cell and developmental biology · 2026Article
- Induction of Nephron-Ductal Dual Lineages via Early Retinoic Acid Signaling Establishes a Platform for Fully Patterned Kidney Organoids.Advanced healthcare materials · 2025Article
- Platelets induce epithelial to mesenchymal transition in renal proximal tubular epithelial cells through TGF-β signaling pathway.Molecular medicine (Cambridge, Mass.) · 2025Article
- Human fetal kidney organoids model early human nephrogenesis and Notch-driven cell fate.The EMBO journal · 2025Article
- Controlling nephron precursor differentiation to generate proximal-biased kidney organoids with emerging maturity.Nature communications · 2025Article
- Bioprinting of Alginate-Norbornene bioinks to create a versatile platform for kidneyBioactive materials · 2025Article
- Multifaceted roles of Epac signaling in renal functions.The Biochemical journal · 2025Review
- Human and rat renal proximal tubule in vitro models for ADME applications.Archives of toxicology · 2025Review
- Unveiling spontaneous renal tubule-like structures from human adult renal progenitor cell spheroids derived from urine.Stem cells translational medicine · 2025Article
- Enhanced Kidney Targeting and Distribution of Tubuloids During Normothermic Perfusion.Transplant international : official journal of the European Society for Organ Transplantation · 2025Article
- Scalable production of uniform and mature organoids in a 3D geometrically-engineered permeable membrane.Nature communications · 2024Article
- Establishment of epithelial inflammatory injury model using adult kidney organoids.Life medicine · 2024Article
- Guidelines for Manufacturing and Application of Organoids: Kidney.International journal of stem cells · 2024Article
- Single-cell guided prenatal derivation of primary fetal epithelial organoids from human amniotic and tracheal fluids.Nature medicine · 2024Article
- Advances and potential of regenerative medicine in pediatric nephrology.Pediatric nephrology (Berlin, Germany) · 2024Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kidney organoids generated from induced pluripotent stem cells (iPSC) have proven valuable for studies of kidney development, disease, and therapeutic screening. However, specific applications have been hampered by limited expansion capacity, immaturity, off-target cells, and inability to access the apical side. Here, we apply recently developed tubuloid protocols to purify and propagate kidney epithelium from d7+18 (post nephrogenesis) iPSC-derived organoids. The resulting 'iPSC organoid-derived (iPSCod)' tubuloids can be exponentially expanded for at least 2.5 mo, while retaining expression of important tubular transporters and segment-specific markers. This approach allows for selective propagation of the mature tubular epithelium, as immature cells, stroma, and undesirable off-target cells rapidly disappeared. iPSCod tubuloids provide easy apical access, which enabled functional evaluation and demonstration of essential secretion and electrolyte reabsorption processes. In conclusion, iPSCod tubuloids provide a different, complementary human kidney model that unlocks opportunities for functional characterization, disease modeling, and regenerative nephrology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.