Evidence map›Paper›PMID 36733254›Full record

ArticleBiophysical journal2023

PDK1:PKCα heterodimer association-dissociation dynamics in single-molecule diffusion tracks on a target membrane.

Moshe T Gordon, Brian P Ziemba, Joseph J Falke

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Membranes in focus.Biophysical journal · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Moshe T GordonMolecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, Colorado.
Brian P ZiembaMolecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, Colorado.
Joseph J FalkeMolecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, Colorado. Electronic address: falke@colorado.edu.
University of Colorado Boulder · US

Funding

Mechanisms of Signaling on Membrane SurfacesR01GM063235 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI FALKE, JOSEPH J · 2001 to 2021
$6.4M
Predoctoral Training Molecular BiophysicsT32GM065103 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI FALKE, JOSEPH J · 2002 to 2021
$4.3M
Mechanisms of Signaling on Membrane SurfacesR35GM144346 · NIGMS · UNIVERSITY OF COLORADO · PI JOSEPH J FALKE · 2022 to 2026
$2.0M
NIGMS NIH HHS R01 GM063235NIGMS NIH HHS R35 GM144346NIGMS NIH HHS T32 GM065103
6 · The paper itself

Abstract

Previous studies have documented the formation of a heterodimer between the two protein kinases PDK1 and PKCα on a lipid bilayer containing their target lipids. This work investigates the association-dissociation kinetics of this PDK1:PKCα heterodimer. The approach monitors the two-dimensional diffusion of single, membrane-associated PDK1 molecules for diffusivity changes as PKCα molecules bind and unbind. In the absence of PKCα, a membrane-associated PDK1 molecule exhibits high diffusivity (or large diffusion constant, D) because its membrane-contacting PH domain binds the target PIP

Indexed as

Lipid BilayersProtein Kinase C-alphaDiffusionProtein BindingSignal TransductionLipid BilayersProtein Kinase C-alpha

Identifiers

PMID36733254
PMCPMC10257113
OpenAlexW4319062740

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.