ArticlePLoS genetics2023
RPG interacts with E3-ligase CERBERUS to mediate rhizobial infection in Lotus japonicus.
Article in PLoS genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Nonnodulating Fagales retain the functional NODULE INCEPTION gene.The New phytologist · 2026Article
- A recently evolved Nodulin recruited by NIN for root hair rhizobial infection in Medicago truncatula.The New phytologist · 2026Article
- Review
- Cyclophilin A-mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes.The New phytologist · 2026Article
- Chromosome-Level Genome and Organ-Specific Transcriptome of Alnus glutinosa Uncover Lineage-Specific Innovations in Root Nodule Symbiosis.Plant, cell & environment · 2026Article
- Ubiquitination as a multi-layer regulatory network in legume-rhizobium symbiosis.Plant cell reports · 2026Review
- The root nodule symbiosis regulator NIN exhibits broad DNA binding specificity conferred by an NLP-inherited motif.Science advances · 2026Article
- Hormone-modulated transcription factors orchestrating the root nodule symbiosis.Frontiers in plant science · 2026Review
- A legume cellulase required for rhizobial infection and colonization in root nodule symbiosis.Nature communications · 2025Article
- Multi-omics profiling of Nanhaia speciosa and Nanhaia fordii: insights into lectin dynamics, nodulation, and triterpenoid saponin biosynthesis.BMC biology · 2025Article
- One versus many independent assemblies of symbiotic nitrogen fixation in flowering plants.Nature communications · 2025Review
- Enacting partner specificity in legume-rhizobia symbioses.aBIOTECH · 2025Review
- Annexin- and calcium-regulated priming of legume root cells for endosymbiotic infection.Nature communications · 2024Article
- Cellular basis of legume-rhizobium symbiosis.Plant communications · 2024Review
- Conserved cis-elements enable NODULES WITH ACTIVATED DEFENSE1 regulation by NODULE INCEPTION during nodulation.The Plant cell · 2024Article
- Article
- NIN-at the heart of NItrogen-fixing Nodule symbiosis.Frontiers in plant science · 2023Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Symbiotic interactions between rhizobia and legumes result in the formation of root nodules, which fix nitrogen that can be used for plant growth. Rhizobia usually invade legume roots through a plant-made tunnel-like structure called an infection thread (IT). RPG (Rhizobium-directed polar growth) encodes a coiled-coil protein that has been identified in Medicago truncatula as required for root nodule infection, but the function of RPG remains poorly understood. In this study, we identified and characterized RPG in Lotus japonicus and determined that it is required for IT formation. RPG was induced by Mesorhizobium loti or purified Nodulation factor and displayed an infection-specific expression pattern. Nodule inception (NIN) bound to the RPG promoter and induced its expression. We showed that RPG displayed punctate subcellular localization in L. japonicus root protoplasts and in root hairs infected by M. loti. The N-terminal predicted C2 lipid-binding domain of RPG was not required for this subcellular localization or for function. CERBERUS, a U-box E3 ligase which is also required for rhizobial infection, was found to be localized similarly in puncta. RPG co-localized and directly interacted with CERBERUS in the early endosome (TGN/EE) compartment and near the nuclei in root hairs after rhizobial inoculation. Our study sheds light on an RPG-CERBERUS protein complex that is involved in an exocytotic pathway mediating IT elongation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.