Evidence mapPaperPMID 36742073Full record

ArticleFrontiers in cardiovascular medicine2023

Growth hormone-releasing hormone agonist attenuates vascular calcification in diabetic db/db mice.

Hao-Lin Ren, Ruiping Cai, Ruize Xue, Yaoxia Zhang, Qian Xu, Xianyang Zhang, RenZhi Cai, Wei Sha, Andrew V Schally, Ming-Sheng Zhou

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cardiovascular medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. GHRH in diabetes and metabolism.Reviews in endocrine & metabolic disorders · 2025
    Review
  6. Effects of GHRH and its analogues on the Vascular System.Reviews in endocrine & metabolic disorders · 2025
    Review
  7. Review
  8. Review
  9. Review
  10. Article
  11. Oxidative Stress, Endothelial Dysfunction, andAntioxidants & redox signaling · 2024
    Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Hao-Lin RenDepartment of Radiology, The First Affiliated Hospital, Dalian Medical University, Dalian, China.
Ruiping CaiScience and Research Center, Shenyang Medical College, Shenyang, China.
Ruize XueScience and Research Center, Shenyang Medical College, Shenyang, China.
Yaoxia ZhangScience and Research Center, Shenyang Medical College, Shenyang, China.
Qian XuScience and Research Center, Shenyang Medical College, Shenyang, China.
Xianyang ZhangVeterans Affairs Medical Center, Endocrine, Polypeptide and Cancer Institute, Miami, FL, United States.
RenZhi CaiVeterans Affairs Medical Center, Endocrine, Polypeptide and Cancer Institute, Miami, FL, United States.
Wei ShaVeterans Affairs Medical Center, Endocrine, Polypeptide and Cancer Institute, Miami, FL, United States.
Andrew V SchallyVeterans Affairs Medical Center, Endocrine, Polypeptide and Cancer Institute, Miami, FL, United States.
Ming-Sheng ZhouScience and Research Center, Shenyang Medical College, Shenyang, China.
Shenyang Medical College · CNFirst Affiliated Hospital of Dalian Medical University · CNSylvester Comprehensive Cancer Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Vascular calcification (VC) is an independent risk factor for cardiovascular diseases. VC increases mortality of all-causes. VC is one of most common cardiovascular complications in type II diabetes. So far, no therapy has been proven to be effective in treatment of clinical VC. The present study investigated the therapeutic effects of MR409, an agonistic analog of growth hormone-releasing hormone (GHRH-A), on VC in diabetic db/db mice. Method and result: Diabetic mice were injected with MR409 subcutaneously every day for 8 weeks. Long-term treatment with MR409 improved serum lipid profile and endothelium-dependent relaxation to acetylcholine, and reduced vascular structural injury in diabetic mice without affecting serum growth hormone level. Echocardiography showed that calcium plaques present in heart valve of diabetic mice disappeared in diabetic mice after treatment with MR409. MR409 inhibited vascular calcium deposition associated with a marked reduction in the expressions of osteogenic-regulated alkaline phosphatase (ALP) and transcription osteogenic marker gene Runx2 in diabetic mice. MR409 also inhibited vascular reactive oxygen species (ROS) generation and upregulated the expressions of anti-calcifying protein Klotho in diabetic mice. Discussion: Our results demonstrate that GHRH-A MR409 can effectively attenuate VC and heart valve calcification, and protect against endothelial dysfunction and vascular injury in diabetic mice without significantly affecting pituitary-growth hormone axis. The mechanisms may involve upregulation of anti-calcifying protein Klotho and reduction in vascular ROS and the expression of redox sensitive osteogenic genes Runx2 and ALP. GHRH-A may represent a new pharmacological strategy for treatment of VC and diabetics associated cardiovascular complications.

Indexed as

diabetesgrowth hormone-releasing hormoneoxidative stressvascular calcificationvascular injury

Identifiers

PMID36742073
PMCPMC9889365
OpenAlexW4317396512

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.