Evidence mapPaperPMID 36768450Full record

ArticleInternational journal of molecular sciences2023

Combination of Radix Astragali and Safflower Promotes Angiogenesis in Rats with Ischemic Stroke via Silencing PTGS2.

Shouchao Xu, Jiehong Yang, Haitong Wan, Li Yu, Yu He

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shouchao XuSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Jiehong YangSchool of Basic Medicine Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Haitong WanSchool of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Li YuSchool of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Yu HeSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.

Funding

National Natural Science Foundation of China Nos. 81873226National Natural Science Foundation of China Nos.81973560
6 · The paper itself

Abstract

Promotion of angiogenesis and restoration of the blood flow in the ischemic penumbra is an effective treatment for patients with ischemic stroke (IS). Radix astragali-safflower (AS), a classic herbal pair for accelerating blood circulation and dispersing blood stasis, has been used for thousands of years to treat patients with IS in China. Even so, the mechanism of the treatment of IS by AS is still undecipherable. In the current study, network pharmacology was firstly employed to unveil the mechanism of AS in treating IS, which showed that AS might promote angiogenesis associated with PTGS2 silence. Middle cerebral artery occlusion/reperfusion (MCAO/R) model rats were then used as the experimental animals to verify the prediction result. The experimental results revealed that treatment with AS improved the cerebral infarct volume, neurological damage, and cerebral histopathological damage; inhibited cell apoptosis; increased the contents of PDGF-BB, EPO, and TGF-β1; and reduced the levels of PF4, Ang-2, and TIMP-1 in serum. Immunohistochemical staining demonstrated that the expression of PTGS2 was dramatically increased in the hippocampus and cerebral cortex of rats with MCAO/R, and this trend was reversed by the treatment of AS. Immunofluorescent staining expressed that AS reversed the down-regulation of VEGF and further promoted the expression of CD31, which indicated that AS promoted angiogenesis in MCAO/R rats. The abnormal protein or mRNA expression of PTGS2, PGI2, bFGF, TSP-1, and VEGF in the penumbra were transposed by AS or Celecoxib (an inhibitor of PTGS2). In conclusion, the protective mechanism of AS for IS promoted angiogenesis and was involved with PTGS2 silence.

Indexed as

Brain InjuriesBrain IschemiaCarthamus tinctoriusIschemic StrokeStrokeAnimalsAstragalus propinquusCyclooxygenase 2Drugs, Chinese HerbalInfarction, Middle Cerebral ArteryRatsRats, Sprague-DawleyVascular Endothelial Growth Factor ACyclooxygenase 2Drugs, Chinese HerbalHuang QiPtgs2 protein, ratVascular Endothelial Growth Factor Aangiogenesisischemic penumbraischemic strokePTGS2Radix astragali and safflower

Identifiers

PMID36768450
PMCPMC9916507

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.