Evidence mapPaperPMID 36769200Full record

ArticleInternational journal of molecular sciences2023

Cytoprotective Effects of Human Platelet Lysate during the Xeno-Free Culture of Human Donor Corneas.

Delia Talpan, Sabine Salla, Linus Meusel, Peter Walter, Chao-Chung Kuo, Julia Franzen, Matthias Fuest

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. From Donation to Innovation: New Blood-Derived Products.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Delia TalpanDepartment of Ophthalmology, RWTH Aachen University, 52074 Aachen, Germany.
Sabine SallaDepartment of Ophthalmology, RWTH Aachen University, 52074 Aachen, Germany.
Linus MeuselDepartment of Ophthalmology, RWTH Aachen University, 52074 Aachen, Germany.
Peter WalterDepartment of Ophthalmology, RWTH Aachen University, 52074 Aachen, Germany.
Chao-Chung KuoGenomics Facility, Interdisciplinary Center for Clinical Research (IZKF), RWTH Aachen University, 52074 Aachen, Germany.ORCID 0000-0001-8879-4754
Julia FranzenGenomics Facility, Interdisciplinary Center for Clinical Research (IZKF), RWTH Aachen University, 52074 Aachen, Germany.
Matthias FuestDepartment of Ophthalmology, RWTH Aachen University, 52074 Aachen, Germany.ORCID 0000-0002-0573-2599
RWTH Aachen University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We evaluated the suitability of 2% human platelet lysate medium (2%HPL) as a replacement for 2% fetal bovine serum medium (2%FBS) for the xeno-free organ culture of human donor corneas. A total of 32 corneas from 16 human donors were cultured in 2%FBS for 3 days (TP1), then evaluated using phase contrast microscopy (endothelial cell density (ECD) and cell morphology). Following an additional 25-day culture period (TP2) in either 2%FBS or 2%HPL, the pairs were again compared using microscopy; then stroma and Descemet membrane/endothelium (DmE) were processed for next generation sequencing (NGS). At TP2 the ECD was higher in the 2%HPL group (2179 ± 288 cells/mm

Indexed as

Cell Culture TechniquesEndothelial CellsBlood PlateletsCell DifferentiationCell ProliferationCells, CulturedCorneaCulture MediaFatty Acid-Binding ProteinsHumansCulture MediaFABP5 protein, humanFatty Acid-Binding Proteinscorneaculturefetal bovine serumfetal calf serumplatelet lysate

Identifiers

PMID36769200
PMCPMC9917909
OpenAlexW4319027505

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.