Evidence map›Paper›PMID 36787055›Full record

ArticleMolecular biology reports2023

Melatonin/nicotinamide mononucleotide/ubiquinol: a cocktail providing superior cardioprotection against ischemia/reperfusion injury in a common co-morbidities modelled rat.

Behnaz Mokhtari, Poul Flemming Høilund-Carlsen, Leila Chodari, Masoud Yasami, Reza Badalzadeh, Samad Ghaffari

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Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Behnaz MokhtariAlavi Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran.ORCID http://orcid.org/0000-0002-3355-9869
Poul Flemming Høilund-CarlsenDepartment of Nuclear Medicine, Odense University Hospital, Odense, Denmark.ORCID http://orcid.org/0000-0001-7420-2367
Leila ChodariNeurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran.
Masoud YasamiStudent Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Reza BadalzadehMolecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. badalzadehr@tbzmed.ac.ir.ORCID http://orcid.org/0000-0002-8092-7820
Samad GhaffariAlavi Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran. ghafaris@gmail.com.ORCID http://orcid.org/0000-0001-6806-9387

Funding

Alavi Aging Research Institute, Tabriz University of Medical Sciences, Tabriz-Iran 65737National Institute for Medical Research Development 978686
6 · The paper itself

Abstract

backgroundThe metabolic and intracellular abnormalities in aging and diabetes cause loss of cardioprotection by routine interventions against myocardial ischemia/reperfusion (I/R) injury. We aimed to evaluate the possible interaction of aging and type-2 diabetes mellitus with cardioprotection and the potential protective effect of a mitochondrial cocktail (melatonin/nicotinamide mononucleotide (NMN)/ubiquinol) on myocardial I/R injury in aged diabetic rats.

methodsMale Wistar rats (n = 108, 22-24 months old, 400-450 g) received high-fat diet/low dose of streptozotocin to induce type-2 diabetes, then were randomized into 9 groups of 12 rats each with/without I/R and/or melatonin, NMN, and ubiquinol, alone or in dual or triple combinations. Myocardial I/R was induced by LAD occlusion for 30 min followed by 24 h reperfusion. NMN (100 mg/kg/48 h, intraperitoneally) was administered for 28 days before I/R operation. Melatonin (10 mg/kg, intraperitoneally) and/or ubiquinol (30 mg/kg, intravenously) were administered at early reperfusion. Finally, hemodynamic index changes, infarct size, CK-MB levels, mitochondrial functional endpoints, and expression of mitochondrial biogenesis genes (SIRT-1/PGC-1α/NRF-2/TFAM) were assessed.

resultsThe solo and dual applications of melatonin, NMN, and ubiquinol did not exert remarkable cardioprotective impacts. However, the triple combination improved myocardial function and decreased infarct size and CK-MB levels following myocardial I/R (P < .05 to P < .01). It also improved mitochondrial function and restored mitochondrial biogenesis genes (P < .01).

conclusionsCombination therapy with melatonin, NMN, and ubiquinol exerted significant cardioprotection and improved mitochondrial function and biogenesis via upregulation of SIRT-1/PGC-1α/NRF-2/TFAM profiles in aged diabetic rats and, thus, offers a promising strategy for providing noticeable cardioprotection against I/R injury also in aged diabetic patients.

Indexed as

Diabetes Mellitus, ExperimentalDiabetes Mellitus, Type 2MelatoninMyocardial IschemiaMyocardial Reperfusion InjuryAnimalsInfarctionIschemiaMaleMorbidityNicotinamide MononucleotideRatsRats, WistarUbiquinoneMelatoninNicotinamide MononucleotideubiquinolUbiquinoneAgingCardioprotectionDiabetesMelatoninNicotinamide mononucleotideUbiquinol

Identifiers

PMID36787055

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.