ArticlePlant methods2023
Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method.
Article in Plant methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Article
- Establishment of an efficient and reliable Agrobacterium-mediated genetic transformation system for the elite male-sterile poplar 'Siyang-1'.BMC plant biology · 2026Article
- The GATA8-GRF5-XTH9 feed-forward loop regulates cell size in poplar.Horticulture research · 2026Article
- Synchronization based on precise cell cycle progression identification to enhance tetraploid induction in '84 K' poplar.Plant cell reports · 2025Article
- Walnut genome editing: an optimized CRISPR/Cas9 platform with superior genotypes and endogenous promoters.Horticulture research · 2025Article
- Efficient induction of tetraploids via adventitious bud regeneration and subsequent phenotypic variation in Acacia melanoxylon.Plant methods · 2025Article
- Establishment of a leaf regeneration system and its molecular basis in Poplar 741.BMC plant biology · 2025Article
- Conquering Limitations: Exploring the Factors that Drive Successful Agrobacterium-Mediated Genetic Transformation of Recalcitrant Plant Species.Molecular biotechnology · 2025Review
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- An Effective Somatic-Cell Regeneration and Genetic Transformation Method Mediated byPlants (Basel, Switzerland) · 2024Article
- An Efficient Transformation System for Fast Production ofPlants (Basel, Switzerland) · 2023Article
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8 authors.
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Abstract
backgroundAgrobacterium tumefaciens-mediated leaf disc genetic transformation is an important way to achieve transgenics or gene editing. Ensuring stable and efficient genetic transformation is still an important problem in modern biology. It is assumed that the difference in the development status of genetic transformation cells of receptor materials is the main reason for the difference and instability of genetic transformation efficiency; the stable and efficient genetic transformation rate can be obtained by defining the appropriate treatment period of the receptor material and applying genetic transformation in a timely manner.
resultsBased on these assumptions, we studied and established an efficient and stable Agrobacterium-mediated plant transformation system with hybrid poplar (Populus alba × Populus glandulosa, 84 K) leaves, stem segments and tobacco leaves as the research objects. There were differences in the development process of leaf bud primordial cells from different explants, and the genetic transformation efficiency was significantly related to the cell development stage of the in vitro cultured materials. Among them, the genetic transformation rate of poplar and tobacco leaves was the highest on the 3rd and 2nd day of culture, reaching 86.6% and 57.3%, respectively. The genetic transformation rate of poplar stem segments was the highest on the 4th day of culture, reaching 77.8%. The best treatment period was from the development of leaf bud primordial cells to the S phase of the cell cycle. The number of cells detected using flow cytometry and 5-ethynyl-2'-deoxyuridine (EdU) staining, the expression of cell cycle-related protein CDKB1; 2, CDKD1; 1, CYCA3; 4, CYCD1; 1, CYCD3; 2, CYCD6; 1, and CYCH; 1 of explants, and morphological changes of explants can be used as indicators to determine the appropriate treatment period for genetic transformation.
conclusionsOur study provides a new and universal set of methods and characteristics to identify the S phase of the cell cycle and apply genetic transformation treatments at the appropriate time. Our results are of great significance for improving the efficiency and stability of plant leaf disc genetic transformation.
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