ArticleNature microbiology2023
Metabolic heterogeneity and cross-feeding within isogenic yeast populations captured by DILAC.
Article in Nature microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Drug-microbiome-host interactions: antimicrobial effects of non-antibiotic compounds.Nature reviews. Gastroenterology & hepatology · 2026Review
- Emergent phenotypic heterogeneity in synthetic cross-feeding consortia driven by heterogeneous metabolite distribution.National science review · 2026Article
- Dissecting autonomous enzymatic variability in single cells.Nature communications · 2026Article
- Single cell profiling framework reveals metabolic subpopulations as drivers of bioproduction heterogeneity.Nature communications · 2025Article
- The Alkane 1-Monooxygenase Gene alkB of Pseudomonas sp. FF2 Is Upregulated During Colonisation of Arabidopsis thaliana Leaves.Environmental microbiology reports · 2025Article
- A transient mutational burst occurs during yeast colony development.Molecular systems biology · 2025Article
- Spatiotemporal development of expanding bacterial colonies driven by emergent mechanical constraints and nutrient gradients.Nature communications · 2025Article
- A dual reporter system for intracellular and extracellular amino acid sensing in budding yeast.Molecular biology of the cell · 2025Article
- The impact of phenotypic heterogeneity on fungal pathogenicity and drug resistance.FEMS microbiology reviews · 2025Review
- Phenotypic heterogeneity follows a growth-viability tradeoff in response to amino acid identity.Nature communications · 2024Article
- Novel antifungals and treatment approaches to tackle resistance and improve outcomes of invasive fungal disease.Clinical microbiology reviews · 2024Review
- Integrative analysis of yeast colony growth.Communications biology · 2024Article
- The characteristics of differentiated yeast subpopulations depend on their lifestyle and available nutrients.Scientific reports · 2024Article
- Cross-feeding promotes heterogeneity within yeast cell populations.Nature communications · 2024Article
- Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p inInternational journal of molecular sciences · 2023Article
- Metabolic exchanges are ubiquitous in natural microbial communities.Nature microbiology · 2023Review
- Diverse mechanisms of bioproduction heterogeneity in fermentation and their control strategies.Journal of industrial microbiology & biotechnology · 2023Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
Genetically identical cells are known to differ in many physiological parameters such as growth rate and drug tolerance. Metabolic specialization is believed to be a cause of such phenotypic heterogeneity, but detection of metabolically divergent subpopulations remains technically challenging. We developed a proteomics-based technology, termed differential isotope labelling by amino acids (DILAC), that can detect producer and consumer subpopulations of a particular amino acid within an isogenic cell population by monitoring peptides with multiple occurrences of the amino acid. We reveal that young, morphologically undifferentiated yeast colonies contain subpopulations of lysine producers and consumers that emerge due to nutrient gradients. Deconvoluting their proteomes using DILAC, we find evidence for in situ cross-feeding where rapidly growing cells ferment and provide the more slowly growing, respiring cells with ethanol. Finally, by combining DILAC with fluorescence-activated cell sorting, we show that the metabolic subpopulations diverge phenotypically, as exemplified by a different tolerance to the antifungal drug amphotericin B. Overall, DILAC captures previously unnoticed metabolic heterogeneity and provides experimental evidence for the role of metabolic specialization and cross-feeding interactions as a source of phenotypic heterogeneity in isogenic cell populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.