Evidence map›Paper›PMID 36798294›Full record

ArticlebioRxiv : the preprint server for biology2023

Female gene networks are expressed in myofibroblast-like smooth muscle cells in vulnerable atherosclerotic plaques.

Ernest Diez Benavente, Santosh Karnewar, Michele Buono, Eloi Mili, Robin J G Hartman, Daniek Kapteijn, Lotte Slenders, Mark Daniels, Redouane Aherrahrou, Tobias Reinberger and 14 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

24 authors at 6 institutions in 5 countries.

Ernest Diez BenaventeLaboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht University, the Netherlands.
Santosh KarnewarRobert M. Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA, USA.
Michele BuonoLaboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht University, the Netherlands.
Eloi MiliLaboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht University, the Netherlands.
Robin J G HartmanLaboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht University, the Netherlands.
Daniek KapteijnLaboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht University, the Netherlands.
Lotte SlendersCentral Diagnostic Laboratory, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
Mark DanielsLaboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht University, the Netherlands.
Redouane AherrahrouCenter for Public Health Genomics, University of Virginia, Charlottesville, VA, USA.
Tobias ReinbergerInstitute for Cardiogenetics, University of Lübeck, Lübeck, Germany.
Barend M MolDepartment of Vascular Surgery, University Medical Centre Utrecht, Utrecht, Utrecht University, The Netherlands.
Gert J de BorstDepartment of Vascular Surgery, University Medical Centre Utrecht, Utrecht, Utrecht University, The Netherlands.
Dominique P V de KleijnDepartment of Vascular Surgery, University Medical Centre Utrecht, Utrecht, Utrecht University, The Netherlands.
Koen H M PrangeDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University' Leiden, The Netherlands.
Marie A C DepuydtDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University' Leiden, The Netherlands.
Menno P J de WintherDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University' Leiden, The Netherlands.
Johan KuiperDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University' Leiden, The Netherlands.
Johan L M BjörkegrenDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Jeanette ErdmannInstitute for Cardiogenetics, University of Lübeck, Lübeck, Germany.
Mete CivelekCenter for Public Health Genomics, University of Virginia, Charlottesville, VA, USA.
Michal MokryCentral Diagnostic Laboratory, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
Gary K OwensRobert M. Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA, USA.
Gerard PasterkampCentral Diagnostic Laboratory, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
Hester M den RuijterLaboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht University, the Netherlands.
Utrecht University · NLLeiden University · NLUniversity of Virginia · USUniversity of Lübeck · DEKarolinska University Hospital · SEUniversity of Eastern Finland · FI

Funding

BASIC CARDIOVASCULAR RESEARCH TRAINING GRANTT32HL007284 · NHLBI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI Brant E Isakson, Gary K Owens · 1985 to 2026
$19.6M
Endothelial Cell to Mesenchymal Cell Transitions Play a Critical Biological Sex- and Aging-Dependent Role in Formation and Maintenance of the Acta2+ Atherosclerotic Lesion Protective Fibrous CapR01HL156849 · NHLBI · UNIVERSITY OF VIRGINIA · PI OWENS, GARY K · 2022 to 2025
$3.2M
IL1beta signaling in SMCpromotes beneficial changes in late stage atherosclerotic lesion pathogenesisR01HL141425 · NHLBI · UNIVERSITY OF VIRGINIA · PI OWENS, GARY K · 2019 to 2022
$3.1M
Defining SMC phenotypes critical in late stage atherosclerosis pathogenesisR01HL136314 · NHLBI · UNIVERSITY OF VIRGINIA · PI OWENS, GARY K · 2018 to 2021
$3.0M
Role of Metabolic Reprogramming in Formation and Maintenance of the Acta2+ Atherosclerotic Lesion Protective Fibrous CapR01HL155165 · NHLBI · UNIVERSITY OF VIRGINIA · PI OWENS, GARY K · 2021 to 2024
$2.9M
NHLBI NIH HHS R01 HL136314NHLBI NIH HHS R01 HL141425NHLBI NIH HHS R01 HL155165NHLBI NIH HHS R01 HL156849NHLBI NIH HHS T32 HL007284
6 · The paper itself

Abstract

Women presenting with coronary artery disease (CAD) more often present with fibrous atherosclerotic plaques, which are currently understudied. Phenotypically modulated smooth muscle cells (SMCs) contribute to atherosclerosis in women. How these phenotypically modulated SMCs shape female versus male plaques is unknown. Here, we show sex-stratified gene regulatory networks (GRNs) from human carotid atherosclerotic tissue. Prioritization of these networks identified two main SMC GRNs in late-stage atherosclerosis. Single-cell RNA-sequencing mapped these GRNs to two SMC phenotypes: a phenotypically modulated myofibroblast-like SMC network and a contractile SMC network. The myofibroblast-like GRN was mostly expressed in plaques that were vulnerable in females. Finally, mice orthologs of the female myofibroblast-like genes showed retained expression in advanced plaques from female mice but were downregulated in male mice during atherosclerosis progression. Female atherosclerosis is driven by GRNs that promote a fibrous vulnerable plaque rich in myofibroblast-like SMCs.

Identifiers

PMID36798294
PMCPMC9934638
OpenAlexW4320040039

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.