Evidence map›Paper›PMID 36808279›Full record

ArticleApplied microbiology and biotechnology2023

Gamma radiation coupled ADP-ribosyl transferase activity of Pseudomonas aeruginosa PE24 moiety.

Radwa N Morgan, Sarra E Saleh, Hala A Farrag, Khaled M Aboshanab

Erratum issuedOpen access · hybridFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Radwa N MorganNational Centre for Radiation Research and Technology (NCRRT), Drug Radiation Research Department, Egyptian Atomic Energy Authority (EAEA), Ahmed El-Zomor Street, Nasr City, 11787, Cairo, Egypt.
Sarra E SalehMicrobiology and Immunology Department, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Abbassia, 11566, Cairo, Egypt.
Hala A FarragNational Centre for Radiation Research and Technology (NCRRT), Drug Radiation Research Department, Egyptian Atomic Energy Authority (EAEA), Ahmed El-Zomor Street, Nasr City, 11787, Cairo, Egypt.
Khaled M AboshanabMicrobiology and Immunology Department, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Abbassia, 11566, Cairo, Egypt. aboshanab2012@pharma.asu.edu.eg.ORCID http://orcid.org/0000-0002-7608-850X
Ain Shams University · EGEgyptian Atomic Energy Authority · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ADP-ribosyl transferase activity of P. aeruginosa PE24 moiety expressed by E. coli BL21 (DE3) was assessed on nitrobenzylidene aminoguanidine (NBAG) and in vitro cultured cancer cell lines. Gene encoding PE24 was isolated from P. aeruginosa isolates, cloned into pET22b( +) plasmid, and expressed in E. coli BL21 (DE3) under IPTG induction. Genetic recombination was confirmed by colony PCR, the appearance of insert post digestion of engineered construct, and protein electrophoresis using sodium dodecyl-sulfate polyacrylamide gel (SDS-PAGE). The chemical compound NBAG has been used to confirm PE24 extract ADP-ribosyl transferase action through UV spectroscopy, FTIR, c

Indexed as

ADP Ribose TransferasesPseudomonas aeruginosaEscherichia coliGamma RaysGuanidines4-nitrobenzylidene aminoguanidineADP Ribose TransferasesGuanidinesADP-ribosylationCombination therapyGamma irradiationNitrobenzylidene aminoguanidine (NBAG)P. aeruginosa PE24 moiety

Identifiers

PMID36808279
PMCPMC10006270
OpenAlexW4321352660

What Socratic holds

Textfull text, public
LicenceCC BY
measurements read196
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.