Evidence map›Paper›PMID 36830956›Full record

ArticleBiomedicines2023

Integration of Ultrastructural and Computational Approaches Reveals the Protective Effect of Astaxanthin against BPA-Induced Nephrotoxicity.

Refaat A Eid, Muhammad Alaa Edeen, Mohamed A Soltan, Mubarak Al-Shraim, Mohamed Samir A Zaki, Saleh M Al-Qahtani, Eman Fayad, Eman T Salem, Waleed K Abdulsahib, Hebatallah Emam and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedicines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 2 countries.

Refaat A EidPathology Department, College of Medicine, King Khalid University, Abha P.O. Box 62529, Saudi Arabia.ORCID 0000-0001-5633-9562
Muhammad Alaa EdeenCell Biology, Histology & Genetics Division, Biology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.ORCID 0000-0001-5280-7736
Mohamed A SoltanDepartment of Microbiology and Immunology, Faculty of Pharmacy, Sinai University, Ismailia 41611, Egypt.ORCID 0000-0003-3030-1659
Mubarak Al-ShraimPathology Department, College of Medicine, King Khalid University, Abha P.O. Box 62529, Saudi Arabia.
Mohamed Samir A ZakiAnatomy Department, College of Medicine, King Khalid University, Abha P.O. Box 62529, Saudi Arabia.ORCID 0000-0003-0010-0506
Saleh M Al-QahtaniDepartment of Child Health, College of Medicine, King Khalid University, Abha P.O. Box 62529, Saudi Arabia.
Eman FayadDepartment of Biotechnology, College of Sciences, Taif University, Taif 21944, Saudi Arabia.ORCID 0000-0003-2916-0254
Eman T SalemDepartment of Basic Science, Faculty of Physical Therapy, Horus University-Egypt, New Damietta 34518, Egypt.
Waleed K AbdulsahibPharmacology and Toxicology Department, College of Pharmacy, Al-Farahidi University, Baghdad 10001, Iraq.
Hebatallah EmamMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Benha 13518, Egypt.ORCID 0000-0002-8194-7903
Hesham M HassanPathology Department, College of Medicine, King Khalid University, Abha P.O. Box 62529, Saudi Arabia.
King Khalid University · SAZagazig University · EGAl-Farahidi UniversityBenha University · EGDamietta University · EGSinai University · EGTaif University · SA

Funding

King Khalid University G.R.P.1-27-43
6 · The paper itself

Abstract

backgroundBisphenol A (BPA) is an environmental contaminant that can induce deleterious organ effects. Human Cytochrome P450 CYP2C9 enzyme belongs to the essential xenobiotic-metabolizing enzymes, producing ROS as a byproduct. Astaxanthin (ATX) is a powerful antioxidant that protects organs and tissues from the damaging effects of oxidative stress caused by various diseases. AIM OF THE STUDY: This study investigated the possible protective impacts of ATX against BPA-induced nephrotoxicity and its underlying mechanism. MATERIALS AND

methodsKidney tissues were isolated and examined microscopically from control, protected, and unprotected groups of rats to examine the potential protective effect of ATX against nephrotoxicity. Moreover, a molecular dynamic (MD) simulation was conducted to predict the performance of ATX upon binding to the active site of P450 CYP2C9 protein receptor as a potential mechanism of ATX protective effect.

resultsImplemented computational methods revealed the possible underlying mechanism of ATX protection; the protective impact of ATX is mediated by inhibiting P450 CYP2C9 through binding to its dimeric state where the RMSF value for apo-protein and ATX-complex system were 5.720.57 and 1.040.41, respectively, implicating the ATX-complex system to have lesser variance in its residues, leading to the prevention of ROS excess production, maintaining the oxidant-antioxidant balance and re-establishing the proper mitochondrial functionality. Furthermore, the experimental methods validated in silico outcomes and revealed that ATX therapy effectively restored the typical histological architecture of pathological kidney tissues.

conclusionsATX prevents BPA-induced nephrotoxicity by controlling oxidative imbalance and reversing mitochondrial dysfunction. These outcomes shed new light on the appropriate use of ATX as a treatment or prophylactic agent for these severe conditions.

Indexed as

antioxidantATXBPACYP2C9dynamicskidneys

Identifiers

PMID36830956
PMCPMC9953522
OpenAlexW4319795661

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.