Evidence map›Paper›PMID 36845170›Full record

ArticleFrontiers in bioengineering and biotechnology2023

A glucose-blue light AND gate-controlled chemi-optogenetic cell-implanted therapy for treating type-1 diabetes in mice.

Chi-Yu Li, Ting Wu, Xing-Jun Zhao, Cheng-Ping Yu, Zi-Xue Wang, Xiao-Fang Zhou, Shan-Ni Li, Jia-Da Li

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In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chi-Yu LiFurong Laboratory, Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, China.
Ting WuFurong Laboratory, Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, China.
Xing-Jun ZhaoFurong Laboratory, Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, China.
Cheng-Ping YuFurong Laboratory, Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, China.
Zi-Xue WangFurong Laboratory, Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, China.
Xiao-Fang ZhouFurong Laboratory, Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, China.
Shan-Ni LiFurong Laboratory, Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, China.
Jia-Da LiFurong Laboratory, Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exogenous insulin therapy is the mainstay treatment for Type-1 diabetes (T1D) caused by insulin deficiency. A fine-tuned insulin supply system is important to maintain the glucose homeostasis. In this study, we present a designed cell system that produces insulin under an AND gate control, which is triggered only in the presence of both high glucose and blue light illumination. The glucose-sensitive GIP promoter induces the expression of GI-Gal4 protein, which forms a complex with LOV-VP16 in the presence of blue light. The GI-Gal4:LOV-VP16 complex then promotes the expression of UAS-promoter-driven insulin. We transfected these components into HEK293T cells, and demonstrated the insulin was secreted under the AND gate control. Furthermore, we showed the capacity of the engineered cells to improve the blood glucose homeostasis through implantation subcutaneously into Type-1 diabetes mice.

Indexed as

AND gatecell-implanted therapychemigeneticoptogenetictype-1 diabetes

Identifiers

PMID36845170
PMCPMC9954140

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.