Evidence map›Paper›PMID 36856209›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[Circular RNA circRSF1 binds to HuR to promote radiation-induced inflammatory phenotype in hepatic stellate cells].

P Zhou, B Cheng, Y Sun, D Wu, Y Chen

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

P ZhouDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
B ChengFirst School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China.
Y SunDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
D WuDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Y ChenDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nanfang Hospital · CNSouthern Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate whether circular RNA circRSF1 regulates radiation-induced inflammatory phenotype of hepatic stellate cells (HSCs) by binding to HuR protein and repressing its function.

methodsHuman HSC cell line LX2 with HuR overexpression or knockdown was exposed to 8 Gy X-ray irradiation, and the changes in the expression of inflammatory factors (IL-1β, IL-6 and TNF-α) were detected by qRT-PCR. The expressions of IκBα and phosphorylation of NF-κB were detected with Western blotting. The binding of circRSF1 to HuR was verified by RNA pull-down assay and RNA-binding protein immunoprecipitation (RIP). The expressions of inflammatory factors, IκBα and the phosphorylation of NF-κB were detected after modifying the interaction between circRSF1 and HuR.

resultsKnockdown of HuR significantly up- regulated the expressions of IL-1β, IL-6 and TNF-α, decreased IκBα expression and promoted NF-κB phosphorylation in irradiated LX2 cells, whereas overexpression of HuR produced the opposite changes (

conclusioncircRSF1 reduces IκBα expression by binding to HuR to promote the activation of NF-κB pathway, thereby enhancing radiation- induced inflammatory phenotype of HSCs.

Indexed as

ELAV-Like Protein 1Hepatic Stellate CellsRNA, CircularHumansInterleukin-6NF-kappa BNF-KappaB Inhibitor alphaNuclear ProteinsPhenotypeRNATrans-ActivatorsTumor Necrosis Factor-alphaELAVL1 protein, humanELAV-Like Protein 1Interleukin-6NF-kappa BNF-KappaB Inhibitor alphaNuclear ProteinsRNARNA, CircularRSF1 protein, humanTrans-ActivatorsTumor Necrosis Factor-alphacircular RNAhepatic stellate cellsHuRinflammatory phenotyperadiation

Identifiers

PMID36856209
PMCPMC9978731
OpenAlexW4322719908

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.