Evidence map›Paper›PMID 36859458›Full record

ArticleScientific reports2023

Potential miRNA biomarkers and therapeutic targets for early atherosclerotic lesions.

Genesio M Karere, Jeremy P Glenn, Ge Li, Ayati Konar, John L VandeBerg, Laura A Cox

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
5.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 23 citations in OpenAlex.

  1. Trial
  2. Review
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  4. Article
  5. Article
  6. Atherosclerosis: A Pathologist's Perspective.Journal of cardiovascular development and disease · 2026
    Review
  7. MicroRNA Regulatory Targets Related to VObioRxiv : the preprint server for biology · 2026
    Article
  8. Review
  9. Review
  10. Article
  11. Article
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  13. Article
  14. Observational
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Genesio M KarereDepartment of Internal Medicine, Section on Molecular Medicine, Center for Precision Medicine, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA. gkarere@wakehealth.edu.
Jeremy P GlennSouthwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, 78227, USA.
Ge LiDepartment of Internal Medicine, Section on Molecular Medicine, Center for Precision Medicine, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.
Ayati KonarDepartment of Internal Medicine, Section on Molecular Medicine, Center for Precision Medicine, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.
John L VandeBergDepartment of Human Genetics, South Texas Diabetes and Obesity Institute, The University of Texas Rio Grande Valley, Brownville, Harlingen, Edinburg, TX, 78520, USA.
Laura A CoxDepartment of Internal Medicine, Section on Molecular Medicine, Center for Precision Medicine, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.
Wake Forest University · USTexas Biomedical Research Institute · USThe University of Texas Rio Grande Valley · US

Funding

The Southwest National Primate Research Center Supplement- Infrastructure improvements of ABSL2 holding areasP51OD011133 · OD · TEXAS BIOMEDICAL RESEARCH INSTITUTE · PI Larry S. Schlesinger · 2012 to 2026
$129.7M
WNV REPLIVAX VACCINE IN NON-HUMAN PRIMATESP51RR013986 · NCRR · TEXAS BIOMEDICAL RESEARCH INSTITUTE · PI KLEIN, SAMUEL · 1999 to 2011
$79.1M
VETERINARY SERVICES--COREP01HL028972 · NHLBI · TEXAS BIOMEDICAL RESEARCH INSTITUTE · PI VANDEBERG, JOHN L · 1985 to 2012
$42.0M
EXTRAMULAR RESEARCH FACILITIES CONSTRUCTIONC06RR017515 · NCRR · SOUTHWEST FOUNDATION FOR BIOMEDICAL RES · PI SHADE, ROBERT E · 2003 to 2003
$3.1M
EXTRAMURAL RES FACILITIES CONSTRC06RR015456 · NCRR · SOUTHWEST FOUNDATION FOR BIOMEDICAL RES · PI VANDEBERG, JOHN L · 2001 to 2001
$1.1M
Identification of Molecular Mechanisms Underlying Early AtherosclerosisK01HL130697 · NHLBI · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI KARERE, GENESIO · 2016 to 2021
$647k
MOLECULAR AND BIOCHEMICAL GENETICS LABORATORY RENOVATIONC06RR013556 · NCRR · SOUTHWEST FOUNDATION FOR BIOMEDICAL RES · PI SHADE, ROBERT E · 1998 to 1998
–
BABOON F-CAGE FACILITY FOR PRIMATE RESEARCH CENTERC06RR014578 · NCRR · SOUTHWEST FOUNDATION FOR BIOMEDICAL RES · PI VANDEBERG, JOHN L · 1999 to 1999
–
American Heart Association-American Stroke Association 4POST18150019NCRR NIH HHS C06 RR013556NCRR NIH HHS C06 RR014578NCRR NIH HHS C06 RR015456NCRR NIH HHS C06 RR017515NCRR NIH HHS P51 RR013986NHLBI NIH HHS K01 HL130697NHLBI NIH HHS P01 HL028972NIH HHS K01HL130697NIH HHS P01HL028972NIH HHS P51 OD011133
6 · The paper itself

Abstract

Identification of potential therapeutic targets and biomarkers indicative of burden of early atherosclerosis that occur prior to advancement to life-threatening unstable plaques is the key to eradication of CAD prevalence and incidences. We challenged 16 baboons with a high cholesterol, high fat diet for 2 years and evaluated early-stage atherosclerotic lesions (fatty streaks, FS, and fibrous plaques, FP) in formalin-fixed common iliac arteries (CIA). We used small RNA sequencing to identify expressed miRNAs in CIA and in baseline blood samples of the same animals. We found 412 expressed miRNAs in CIA and 356 in blood samples. Eight miRNAs (miR-7975, -486-5p, -451a, -191-5p, -148a-3p, -17-5p, -378c, and -144-3p) were differentially expressed between paired fatty streak lesion and no-lesion sites of the tissue, and 27 miRNAs (e.g., miR-92a-3p, -5001, -342-3p, miR-28-3p, -21-5p, -221-3p, 146a-5p, and -16-5p) in fibrous plaques. The expression of 14 blood miRNAs significantly correlated with extent of lesions and the number of plaques. We identified coordinately regulated miRNA-gene networks in which miR-17-5p and miR-146a-5p are central hubs and miR-5001 and miR-7975 are potentially novel miRNAs associated with early atherosclerosis. In summary, we have identified miRNAs expressed in lesions and in blood that correlate with lesion burden and are potential therapeutic targets and biomarkers. These findings are a first step in elucidating miRNA regulated molecular mechanisms that underlie early atherosclerosis in a baboon model, enabling translation of our findings to humans.

Indexed as

AtherosclerosisMicroRNAsAnimalsAorta, AbdominalBiomarkersDiet, High-FatHumansPapioPlaque, AmyloidBiomarkersMicroRNAs

Identifiers

PMID36859458
PMCPMC9977938
OpenAlexW4322739171

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.